The global outbreak of monkeypox in 2022 challenged the means of early, rapid, and accurate screening of the disease to avoid widespread outbreaks. However, as the most easy and convenient point-of-care testing (POCT) method, lateral flow immunoassay (LFA) is limited by the insufficient sensitivity and low reliability of visualizing single-signal patterns. Here, a triple-signal mode LFA (TLFA) using “four-in-one” multifunctional Au/Pt co-decorated Fe3O4 (MS@Pt) nanotags is reported. The MS@Pt-DTNB tags with excellent magnetic, optical, peroxidase-like, and SERS properties outputs a triple-signal readout of colorimetric, catalytically enhanced colorimetric, and SERS signals for Monkeypox virus (MPXV) detection after magnetic separation. The qualitative and quantitative limits of detection (LODs) of MS@Pt-TLFA strips were 0.005 and 0.0016 ng mL−1 for MPXV A29L protein, respectively, and 73.78 and 17.724 pfu mL−1 for inactivated virus, respectively, which is 2–3 orders of magnitude more sensitive compared with the AuNP-based LFA. The diversity, complementarity, and mutual corroboration of integrated output signals in this MS@Pt-TLFA improve the flexibility, accuracy, and practicability of MPXV detection, providing a potential avenue for immunodiagnostic in a variety of application scenarios.

中文翻译：

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“四合一”多功能纳米杂化物集成磁性比色催化SERS驱动的侧流免疫分析用于MPXV的超灵敏检测


2022 年全球爆发的猴痘疫情对早期、快速、准确筛查该疾病以避免大范围爆发的方法提出了挑战。然而，作为最简单、方便的即时检测（POCT）方法，侧流免疫分析（LFA）由于单信号模式可视化的灵敏度不足和可靠性低而受到限制。在此，报道了一种使用“四合一”多功能 Au/Pt 共装饰 Fe3O4 (MS@Pt) 纳米标签的三信号模式 LFA (TLFA)。 MS@Pt-DTNB 标签具有出色的磁性、光学、类过氧化物酶和 SERS 特性，可输出比色、催化增强比色和 SERS 信号的三重信号读数，用于磁分离后猴痘病毒 (MPXV) 检测。 MS@Pt-TLFA 试纸条的 MPXV A29L 蛋白的定性和定量检测限 (LOD) 分别为 0.005 和 0.0016 ng mL−1，对于灭活病毒分别为 73.78 和 17.724 pfu mL−1，即 2–与基于 AuNP 的 LFA 相比，灵敏度提高了 3 个数量级。 MS@Pt-TLFA 中集成输出信号的多样性、互补性和相互佐证性提高了 MPXV 检测的灵活性、准确性和实用性，为各种应用场景中的免疫诊断提供了潜在的途径。