Metazoans rely on interactions with microorganisms through multiple life stages. For example, developmental trajectories of mosquitoes can vary depending on the microorganisms available during their aquatic larval phase. However, the role that the local environment plays in shaping such host-microbe dynamics and the consequences for the host organism remain inadequately understood. Here, we examine the influence of abiotic factors, locally available bacteria, and their interactions on the development and associated microbiota of the mosquito Aedes albopictus. Our findings reveal that leaf detritus infused into the larval habitat water, sourced from native Hawaiian tree “ōhi‘a lehua Metrosideros polymorpha, invasive strawberry guava Psidium cattleyanum, or a pure water control, displayed a more substantial influence than either temperature variations or simulated microbial dispersal regimes on bacterial community composition in adult mosquitoes. However, specific bacteria exhibited divergent patterns within mosquitoes across detrital infusions which did not align with their abundance in the larval habitat. Specifically, we observed a higher relative abundance of a Chryseobacterium sp. strain in mosquitoes from the strawberry guava infusion than the pure water control, whereas the opposite trend was observed for a Pseudomonas sp. strain. In a follow-up experiment, we manipulated the presence of these two bacterial strains and found larval developmental success was enhanced by including the Chryseobacterium sp. strain in the strawberry guava infusion and the Pseudomonas sp. strain in the pure water control. Collectively, these data suggest that interactions between abiotic factors and microbes of the larval environment can help shape mosquito populations” success.

中文翻译：

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非生物因子塑造蚊子微生物组，促进宿主发育


后生动物在多个生命阶段依赖于与微生物的相互作用。例如，蚊子的发育轨迹可能因水生幼虫阶段可用的微生物而异。然而，当地环境在塑造这种宿主微生物动态中的作用以及对宿主生物体的影响仍然不充分。在这里，我们研究了非生物因素、当地可用的细菌及其相互作用对白纹伊蚊发育和相关微生物群的影响。我们的研究结果表明，注入幼虫栖息地水中的叶子碎屑，来自夏威夷本地树木 “ōhi'a lehua Metrosideros polymorpha、入侵草莓番石榴 Psidium cattleyanum 或纯水控制，对成年蚊子的细菌群落组成的影响比温度变化或模拟微生物扩散机制更显着。然而，特定细菌在蚊子体内通过碎屑输液表现出不同的模式，这与它们在幼虫栖息地中的丰度不一致。具体来说，我们观察到草莓番石榴输液中蚊子中 Chryseobacterium sp. 菌株的相对丰度高于纯水对照，而假单胞菌属菌株则观察到相反的趋势。在后续实验中，我们操纵了这两种细菌菌株的存在，发现通过在草莓番石榴输液中加入 Chryseobacterium sp. 菌株和在纯水对照中加入 Pseudomonas sp. 菌株，可以提高幼虫发育的成功率。总的来说，这些数据表明，非生物因素与幼虫环境微生物之间的相互作用有助于塑造蚊子种群的成功。