This study investigates the biochemical methane potential (BMP) of microalgal biomass, introducing a novel cells disruption method using quartz powder (SiO2). A two-phase algae cultivation, involving nitrogen deprivation and salinity shifts, was employed to biochemically modify the biomass of two brackish green algae strains, Chlorella vulgaris and Monoraphidium contortum, enhancing their methane (CH4) production potential. Mechanical disruption of the algal cells further increased BMP, with C. vulgaris yielding 305 mL CH4/g volatile solids (VS) and M. contortum reaching 324 mL CH4/g VS, reflecting respective increases of 51 % and 86 %. The integration of this efficient mechanical cell disruption method with a simple, stress-based cultivation strategy presents significant potential for enhancing the methane yield of microalgal biomass.

中文翻译：

---

提高微藻的甲烷产量：石英粉的非生物胁迫和细胞破坏


本研究研究了微藻生物质的生化甲烷潜力 (BMP)，引入了一种使用石英粉 (SiO2) 的新型细胞破碎方法。采用涉及氮剥夺和盐度变化的两阶段藻类培养，对两种微咸绿藻菌株（小球藻和Monoraphidium contortum）的生物量进行生化改造，提高其甲烷（CH4）生产潜力。对藻细胞的机械破坏进一步增加了 BMP，其中 C. vulgaris 产生了 305 mL CH4/g 挥发性固体 (VS)，M. contortum 达到了 324 mL CH4/g VS，分别增加了 51% 和 86%。这种高效的机械细胞破碎方法与简单的基于压力的培养策略的结合，为提高微藻生物质的甲烷产量提供了巨大的潜力。