The implementation of environmentally friendly and sustainable remediation strategies positively impacts solid waste management. In this study, the Kocuria marina H-2 and Pseudomonas putida B6-2 co-culture system demonstrated enhanced naphthalene biodegradation efficiency compared to single-strain cultures. Under optimal conditions of 35 °C, 200 rpm/min, and a 1:1 ratio of the co-culture system, the naphthalene biodegradation potential was further increased. Notably, the addition of both ethylenediamine-pretreated lignin and p-hydroxybenzoic acid significantly elevated naphthalene degradation rates to 68.5 %. In addition, the oil-liquid surface tension decreased, while cell surface hydrophobicity and colony-forming units increased with the addition of lignin-derived compounds. The modification of naphthalene bioavailability by ethylenediamine-pretreated lignin would accelerate the uptake and transport of hydrocarbons via ABC transporters and flagellar assembly. Importantly, genes related to bacterial chemotaxis and fatty acid biosynthesis were upregulated during the co-metabolism of naphthalene and p-hydroxybenzoic acid, further enhancing naphthalene bioconversion.

中文翻译：

---

Kocuria marina H-2 和恶臭假单胞菌 B6-2 共培养辅助木质素衍生化合物增强萘生物降解


实施环保和可持续的修复策略对固体废物管理产生积极影响。在这项研究中，与单菌株培养物相比，Kocuria marina H-2 和恶臭假单胞菌 B6-2 共培养系统表现出更高的萘生物降解效率。在35℃、200rpm/min、1:1比例的共培养系统的最佳条件下，萘的生物降解潜力进一步提高。值得注意的是，添加乙二胺预处理的木质素和对羟基苯甲酸可将萘降解率显着提高至 68.5%。此外，随着木质素衍生化合物的添加，油液表面张力降低，而细胞表面疏水性和集落形成单位增加。乙二胺预处理的木质素对萘生物利用度的改变将通过 ABC 转运蛋白和鞭毛组装加速碳氢化合物的吸收和运输。重要的是，在萘和对羟基苯甲酸的共代谢过程中，与细菌趋化性和脂肪酸生物合成相关的基因上调，进一步增强了萘的生物转化。