This study investigated the use of C1 and C2 products synthesized through artificial photosynthesis to enhance the growth and synthesis of pigments, starches, and lipids of Chlorella pyrenoidosa. Compared with the control group, potassium acetate and ethanol significantly promoted the growth of microalgae, while methanol and potassium formate showed certain inhibitory effects on the growth of microalgae, indicating that C2 products were more conducive to the growth of microalgae. It is worth noting that the ability of microalgae to utilize potassium formate after domestication was significantly enhanced, indicating that domestication treatment of microalgae is an effective means to improve the utilization rate of C1 products. Both C1 and C2 products significantly promoted the accumulation of lipid and starch in microalgae cells, and C2 products significantly increased the lipid and starch yield of microalgae, with potassium acetate being the most effective. Under the treatment of 5 g/L potassium acetate, biomass productivity of C. pyrenoidosa reached 0.28 g/L d⁻¹, while starch and lipid yield reached 17.2 mg/L d⁻¹ and 25.2 mg/L d⁻¹, respectively. Transcriptomic analysis revealed significant upregulation of genes involved in photosynthesis, energy metabolism and other elated pathways under the influence of different organics. Furthermore, the addition of potassium acetate and formate reduced the dependency of microalgae on photosynthesis, while ethanol and potassium acetate significantly accelerated the energy metabolism of microalgae, establishing the basis for microalgae growth and biomass production. In conclusion, potassium acetate was found to be a relatively suitable product of artificial photosynthesis for enhancing the production of high-value bioproducts by mixotrophic microalgae, and when C1 products were used for microalgae culture, pretreatment such as microalgae domestication was particularly important.

中文翻译：

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混合营养微藻 小球藻将 C1 和 C2 人工光合作用产物生物转化为高价值生物制品


本研究调查了通过人工光合作用合成的 C1 和 C2 产物的用途，以增强小球藻类杂草色素、淀粉和脂质的生长和合成。与对照组相比，乙酸钾和乙醇显著促进了微藻的生长，而甲醇和甲酸钾对微藻的生长表现出一定的抑制作用，表明 C2 产物更有利于微藻的生长。值得注意的是，微藻驯化后利用甲酸钾的能力显著增强，表明微藻的驯化处理是提高 C1 产品利用率的有效手段。C1 和 C2 产物均显著促进微藻细胞中脂质和淀粉的积累，C2 产物显著提高微藻的脂质和淀粉产量，其中乙酸钾最有效。在 5 g/L 乙酸钾处理下，核糖菌的生物量生产力达到 0.28 g/L ^d-1，而淀粉和脂质产量分别达到 17.2 mg/L ^d-1 和 25.2 mg/L ^d-1。转录组学分析显示，在不同有机物的影响下，参与光合作用、能量代谢和其他兴奋途径的基因显著上调。此外，乙酸钾和甲酸盐的添加降低了微藻对光合作用的依赖性，而乙醇和乙酸钾显着加速了微藻的能量代谢，为微藻生长和生物质生产奠定了基础。 综上所述，发现乙酸钾是相对合适的人工光合作用产物，用于增强混合营养微藻生产高价值生物制品，当 C1 产物用于微藻培养时，微藻驯化等预处理尤为重要。