It is well known that the overall quality of japonica/geng rice is superior to that of indica/xian rice varieties. However, the molecular mechanisms underlying the quality disparities between these two subspecies of rice are still largely unknown. In this study, we have pinpointed a gene homologous to SLR1, termed LCG1, exhibiting significant expression during early caryopsis development and playing a specific role in regulating rice chalkiness and taste by affecting the accumulation of grain storage components, starch granule structure and chain length distribution of amylopectin. LCG1 physically interacts with OsBP5 and indirectly influences the expression of the amylose synthesis gene Waxy (Wx) by hindering the transcriptional activity of the OsBP5/OsEBP89 complex. Notably, sequence variations in the promoter region of LCG1 result in enhanced transcription in japonica rice accessions. This leads to elevated LCG1 expression in CSSL-LCG1^Nip, thereby enhancing rice quality. Our research elucidates the molecular mechanism underlying the impact of the LCG1-OsBP5/OsEBP89-Wx regulatory pathway on rice chalkiness and taste quality, offering new genetic resources for improving the indica rice quality.

中文翻译：

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LCG1-OsBP5/OsEBP89-Wx 模块调节稻米的垩白度和口感品质


众所周知，粳稻/耿稻的整体品质优于籼稻/籼稻品种。然而，这两个水稻亚种之间质量差异的分子机制仍然很大程度上未知。在本研究中，我们找到了一个与SLR1同源的基因，称为LCG1 ，该基因在颖果早期发育过程中表现出显着的表达，并通过影响储粮成分的积累、淀粉颗粒结构和链长分布来调节水稻的垩白度和口感。支链淀粉。 LCG1 与 OsBP5 发生物理相互作用，并通过阻碍 OsBP5/OsEBP89 复合物的转录活性来间接影响直链淀粉合成基因Waxy ( Wx ) 的表达。值得注意的是， LCG1启动子区域的序列变异导致粳稻品种的转录增强。这导致 CSSL -LCG1 ^Nip中LCG1表达升高，从而提高稻米品质。我们的研究阐明了LCG1-OsBP5/OsEBP89 -Wx调控途径对水稻垩白度和口感品质影响的分子机制，为提高籼稻品质提供新的遗传资源。