BackgroundThrombocytopenia, common in preterm newborns, may increase bleeding risk and is often treated with transfusions. Recent studies reveal that transfusing platelets at a high threshold worsens outcomes, possibly due to a “developmental mismatch” between adult‐derived platelets and neonatal hemostatic system. Cord blood‐derived platelet concentrates (CBPCs) could be an alternative for newborns. Our study aims to produce and evaluate the quality parameters of CBPCs during storage.Study Design and MethodsCord blood was collected from placentas after near‐term and full‐term pregnancies. Several production methods were attempted to obtain CBPCs, varying centrifugation settings, filtration, and dilution procedures. Adult‐derived platelet concentrates (PCs) processed with the same methods, and standard PCs from five buffy‐coats were used as controls. Storage tests were performed on days 2, 4–5, 7 from the collection.ResultsCBPCs parameters were compared with adult‐derived PCs, and no significant differences were found for mean platelet volume (MPV), swirling, morphology, glucose, lactate, pCO2, and pO2. pH and bicarbonate were lower in CBPCs. Some significant differences between methods in CD62P expression and JC‐1 ratio were observed. Compared with standard PCs, CBPCs showed lower platelet concentration, pH, and JC‐1. Additionally, both in CBPCs as well as in control PCs, the apoptosis marker phosphatidylserine was elevated.DiscussionCBPCs were of comparable quality to control PCs during storage. However, apoptosis markers in both groups were elevated, suggesting processing and storage of low volumes of PCs require further optimization. Also, filtration of low volumes leads to significant platelet loss, an issue that requires remedy.

中文翻译：

---

使用三种不同方法制备的脐带血浓缩血小板的储存特性


背景血小板减少症常见于早产儿，可能会增加出血风险，通常通过输血进行治疗。最近的研究表明，高阈值输注血小板会导致结果恶化，这可能是由于成人来源的血小板与新生儿止血系统之间的“发育不匹配”。脐带血衍生血小板浓缩物（CBPC）可能是新生儿的替代品。我们的研究旨在产生和评估储存期间 CBPC 的质量参数。研究设计和方法从近足月和足月妊娠后的胎盘中采集脐带血。尝试了多种生产方法来获得 CBPC，包括不同的离心设置、过滤和稀释程序。采用相同方法处理的成人衍生血小板浓缩物 (PC) 和来自五层血沉棕黄层的标准 PC 作为对照。在收集后的第 2、4-5、7 天进行储存测试。结果将 CBPC 参数与成人来源的 PC 进行比较，发现平均血小板体积 (MPV)、漩涡、形态、葡萄糖、乳酸、pCO2 没有显着差异和 pO2。 CBPC 中的 pH 值和碳酸氢盐较低。观察到不同方法在 CD62P 表达和 JC-1 比率方面存在一些显着差异。与标准 PC 相比，CBPC 显示出较低的血小板浓度、pH 和 JC-1。此外，在 CBPC 和对照 PC 中，凋亡标记物磷脂酰丝氨酸均升高。讨论 CBPC 在储存期间与对照 PC 的质量相当。然而，两组的细胞凋亡标记物均升高，表明少量 PC 的处理和存储需要进一步优化。此外，低容量过滤会导致大量血小板损失，这是一个需要补救的问题。