Cell sorting holds broad applications in fields such as early cancer diagnosis, cell differentiation studies, drug screening, and single-cell sequencing. However, achieving high-throughput and high-purity in label-free single-cell sorting is challenging. To overcome this issue, we propose a label-free, high-throughput, and high-accuracy impedance-activated cell sorting system based on impedance detection and dual membrane pumps. Leveraging the low-latency characteristics of FPGA, the system facilitates real-time dual-frequency single-cell impedance detection with high-throughput (5 × 10⁴ cells per s) for HeLa, MDA-MB-231, and Jurkat cells. Furthermore, the system accomplishes low-latency (less than 0.3 ms), label-free, high-throughput (1000 particles per s) and high-accuracy (almost 99%) single-particle sorting using FPGA-based high-precision sort-timing prediction. In experiments with Jurkat and MDA-MB-231 cells, the system achieved a throughput of up to 1000 cells per s, maintaining a pre-sorting purity of 28.57% and increasing post-sorting purity to 97.09%. These findings indicate that our system holds significant potential for applications in label-free, high-throughput cell sorting.

中文翻译：

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无标记高通量阻抗激活细胞分选


细胞分选在早期癌症诊断、细胞分化研究、药物筛选和单细胞测序等领域具有广泛的应用。然而，在无标记单细胞分选中实现高通量和高纯度具有挑战性。为了克服这个问题，我们提出了一种基于阻抗检测和双膜泵的无标记、高通量和高精度阻抗激活细胞分选系统。该系统利用 FPGA 的低延迟特性，以高通量（每秒 5 ×^{10 4} 个细胞）促进 HeLa、MDA-MB-231 和 Jurkat 细胞的实时双频单细胞阻抗检测。此外，该系统使用基于 FPGA 的高精度分选时间预测，实现了低延迟（小于 0.3 毫秒）、无标记、高通量（每秒 1000 个颗粒）和高精度（几乎 99%）的单粒子分选。在 Jurkat 和 MDA-MB-231 细胞的实验中，该系统实现了高达 1000 个细胞/秒的通量，保持了 28.57% 的分选前纯度，并将分选后纯度提高到 97.09%。这些发现表明，我们的系统在无标记、高通量细胞分选方面具有巨大的应用潜力。