Thermophilic fungi are superlative microorganisms for enzyme production, especially cellulase, and their using in biotechnological applications is due to their stability at utmost temperatures. In the current investigation, we isolated six genera encompassing six fungal species and one species variety from 30 samples of compost at 45 °C and 55 °C. Thermomyces lanuginosus was the most rampant species. The colony diameter of T. lanuginosus ranged from 2.8 to 4.3 cm at 45 °C on yeast-starch agar (YpSs) medium with white or greyish-brown mycelia. Fifteen isolates of T. lanuginosus were cellulase producers with variable competencies with a C/Z range of 1.09–1.38 cm. Fascinatingly, the clear zone diameter was much bigger when using Iodine than those obtained using Congo red. T. lanuginosus isolate no. 33 produced substantial amounts of cellulase on the four used media: Corncob (CC), Corncob treated with NaOH (C-NA), Yeast Peptone Dextrose (YPD), and CarboxyMethyl Cellulose (CMC) with the highest activity on CC; 143.9 μg/min, also cellulase gene expression levels of cel6Aq, cel7Aq, and bgl3Aq genes exhibited higher fold changes in the CC condition (7.26-fold, 11.51-fold, and 3.39-fold, respectively). X-ray fluorescence (XRF) analysis revealed the presence of 11 minerals with higher concentrations in CC than in C-NA. Supplementation of corncob medium with rosemary essential oil (CR) completely inhibited cellulase production. It adversely affected the growth, and changes in conidia, which were depicted using a scanning electron microscope (SEM). Interestingly, the conidia appeared much bigger than other media, and the large conidia diameter was 10.2–12.1 μm.

中文翻译：

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从堆肥中分离出的嗜热疏棉状嗜热丝孢菌中的纤维素酶基因表达


嗜热真菌是生产酶（尤其是纤维素酶）的最高级微生物，它们在生物技术应用中的使用是由于它们在最高温度下的稳定性。在当前的调查中，我们从 45 °C 和 55 °C 的 30 个堆肥样品中分离出了 6 个属，包括 6 种真菌和 1 个品种。疏棉状嗜热霉菌是最猖獗的物种。 45 °C 时，在具有白色或灰棕色菌丝体的酵母淀粉琼脂 (YpSs) 培养基上，T. lanuginosus 的菌落直径范围为 2.8 至 4.3 cm。 T. lanuginosus 的 15 个分离株是具有不同能力的纤维素酶生产者，C/Z 范围为 1.09–1.38 cm。令人着迷的是，使用碘获得的透明区域直径比使用刚果红获得的透明区域直径大得多。 T. lanuginosus 分离株编号33 在四种使用的培养基上产生了大量的纤维素酶：玉米芯 (CC)、用 NaOH 处理的玉米芯 (C-NA)、酵母蛋白胨葡萄糖 (YPD) 和羧甲基纤维素 (CMC)，在 CC 上活性最高； 143.9 μg/min，cel6Aq、cel7Aq 和 bgl3Aq 基因的纤维素酶基因表达水平在 CC 条件下也表现出更高的倍数变化（分别为 7.26 倍、11.51 倍和 3.39 倍）。 X 射线荧光 (XRF) 分析显示，CC 中存在 11 种矿物质，其浓度高于 C-NA。在玉米芯培养基中添加迷迭香精油（CR）可完全抑制纤维素酶的产生。它对分生孢子的生长和变化产生不利影响，这是使用扫描电子显微镜（SEM）描绘的。有趣的是，分生孢子比其他培养基大得多，大分生孢子直径为10.2-12.1μm。