Methyl jasmonate (MeJA) is widely involved in the life processes of plants, especially playing an active role in regulating the synthesis and accumulation of metabolites. Prickly ash (Zanthoxylum bungeanum), as a species of spice tree, is rich in flavonoids throughout its entire plant, showing enormous potential for development into functional foods. Flavonoids play crucial roles in signal transduction and defense mechanisms between plants and their environment, influencing the ecological adaptability of plants. Analyzing the regulatory relationship between methyl jasmonate and flavonoid metabolites will help understand the self-protection mechanism of Zanthoxylum bungeanum under adverse stress. Research results show that the upstream promoter of CHSs (Chalcone synthase), a key gene for flavonoid synthesis, contains multiple methyl jasmonate response elements. Exogenous MeJA induction experiments proved that MeJA can significantly increase the expression levels of ZbCHS71, ZbCHS43 and ZbCHS26 in different tissues of Zanthoxylum bungeanum and directly promote the synthesis and accumulation of flavonoid metabolites. The research results help analyze the regulatory relationship between methyl jasmonate and flavonoids, and provide a reference for research on the accumulation of protective substances under stress conditions in Zanthoxylum bungeanum.

茉莉酸甲酯（MeJA）广泛参与植物的生命过程，特别是在调节代谢物的合成和积累方面发挥着积极作用。花椒（ Zanthoxylum bungeanum ）作为香料树种，全株富含黄酮类化合物，具有开发功能性食品的巨大潜力。黄酮类化合物在植物与其环境之间的信号转导和防御机制中发挥着至关重要的作用，影响着植物的生态适应性。分析茉莉酸甲酯与黄酮类代谢产物的调控关系，有助于了解花椒在逆境胁迫下的自我保护机制。研究结果表明，类黄酮合成的关键基因CHS（查尔酮合酶）的上游启动子含有多个茉莉酸甲酯反应元件。外源MeJA诱导实验证明，MeJA能显着提高花椒不同组织中ZbCHS71 、 ZbCHS43和ZbCHS26的表达水平，直接促进黄酮代谢物的合成和积累。研究结果有助于解析茉莉酸甲酯与黄酮类化合物的调控关系，为花椒在逆境条件下保护物质积累的研究提供参考。