The development of new techniques for the effective use of smaller cuttings for cuttage to eliminate the need for exogenous plant growth regulators is expected to enhance the production of chrysanthemum, an important ornamental, edible, and medicinal plant. This study aimed to explore the effect of chlorine dioxide (ClO₂) at promoting cuttage in three varieties of chrysanthemum. Irrigation with 0.005—0.01 mg·L⁻¹ ClO₂ remarkably promoted rooting, as exhibited by their increased biomass, root number, root length, chlorophyll content, and activities of antioxidant enzymes and tryptophan synthase (P < 0.05). A targeted metabolic analysis indicated that the contents of both tryptophan and its derivative indole-3-acetic acid were enhanced significantly under treatment with 0.005 and 0.01 mg·L⁻¹ ClO₂. A total of 584 differentially expressed genes were detected between the CK and 0.01 mg·L⁻¹ ClO₂ treatment as shown by a transcriptomic analysis, which are primarily associated with tryptophan, fatty acid and carbon metabolism. An elemental analysis suggested that treatment with 0.01 mg·L⁻¹ ClO₂ disrupts the imbalance of chemical elements in chrysanthemum cuttings. These results guide the conclusion that suitable concentrations of ClO₂ act as growth stimulants that can improve the cuttage efficiency in chrysanthemum.

开发有效利用较小插条进行扦插的新技术，以消除对外源植物生长调节剂的需求，有望提高菊花的产量，菊花是一种重要的观赏、食用和药用植物。本研究旨在探讨二氧化氯（ClO ₂ ）对三个菊花品种的扦插促进作用。 0.005～0.01 mg·L ^-1 ClO ₂灌溉显着促进生根，其生物量、根数、根长、叶绿素含量以及抗氧化酶和色氨酸合酶活性均增加( P < 0.05)。靶向代谢分析表明，0.005和0.01 mg·L ^-1 ClO ₂处理后，色氨酸及其衍生物吲哚-3-乙酸的含量均显着升高。转录组分析显示CK与0.01 mg·L ^-1 ClO ₂处理之间共检测到584个差异表达基因，主要与色氨酸、脂肪酸和碳代谢相关。元素分析表明，0.01 mg·L ^-1 ClO ₂处理破坏了菊花插条中化学元素的不平衡。这些结果得出这样的结论：适当浓度的ClO ₂作为生长刺激剂可以提高菊花的扦插效率。