3D printed gelatin methacryloyl hydrogels for perfusion culture of human trabecular meshwork cells and glaucoma studies,Biotechnology and Bioengineering

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3D printed gelatin methacryloyl hydrogels for perfusion culture of human trabecular meshwork cells and glaucoma studies
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2024-09-18 , DOI: 10.1002/bit.28849
Bikram Adhikari ₁ , Prasanga Barakoti ₁ , Mina B Pantcheva ₂ , Melissa D Krebs _{1,

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Affiliation

Glaucoma, a progressive eye disease leading to irreversible blindness, currently affects over 70 million people globally. Elevated intraocular pressure (IOP) is implicated in its development. IOP is carefully regulated by the trabecular meshwork (TM). However, studying TM behavior has been limited to traditional tissue culture studies or costly ex vivo cultures of animal and donor eyes. Developing novel functional TM models could enhance cell/tissue behavior understanding and aid therapeutic development for glaucoma. In this study, we 3D printed a simplified and reproducible model of the human TM (hTM) and studied hTM cell behavior under static and dynamic cultures. Gelatin Methacryloyl bioinks proved suitable for printing with viable and proliferative hTM cells expressing crucial marker genes in response to glucocorticoid induction. This, to our knowledge, is the first functional 3D printed hTM model and aims to facilitate TM research. Moreover, this easily reproducible model could also be applicable in the study of numerous other cell types throughout the body.

中文翻译：

3D 打印明胶甲基丙烯酰水凝胶，用于人小梁网细胞的灌注培养和青光眼研究

青光眼是一种导致不可逆失明的进行性眼病，目前影响着全球超过 7000 万人。眼压升高（IOP）与其发展有关。IOP 由小梁网（TM）仔细调节。然而，研究 TM 行为仅限于传统的组织培养研究或昂贵的动物和供体眼睛的离体培养。开发新的功能性 TM 模型可以增强对细胞/组织行为的理解，并有助于青光眼的治疗开发。在这项研究中，我们 3D 打印了人类 TM （hTM）的简化且可重复的模型，并研究了静态和动态培养下的 hTM 细胞行为。明胶甲基丙烯酰生物墨水被证明适用于打印表达响应糖皮质激素诱导的关键标记基因的活细胞和增殖性 hTM 细胞。据我们所知，这是第一个功能性 3D 打印的 hTM 模型，旨在促进 TM 研究。此外，这种易于重复的模型也适用于研究全身许多其他细胞类型。

更新日期：2024-09-18

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