Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Design Optimization of a Novel Catalytic Approach for Transglucosylated Isomaltooligosaccharides into Dietary Polyols Structures by Leuconostoc mesenteroides Dextransucrase
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-18 , DOI: 10.1021/acs.jafc.4c04222
Ana Muñoz-Labrador ₁ , Elisa G Doyagüez ₂ , Silvana Azcarate ₃ , Cristina Julio-Gonzalez ₁ , Daniela Barile ₄ , F Javier Moreno ₁ , Oswaldo Hernandez-Hernandez _{1,

4}

Affiliation

Polyols, or sugar alcohols, are widely used in the industry as sweeteners and food formulation ingredients, aiming to combat the incidence of diet-related Non-Communicable Diseases. Given the attractive use of Generally Regarded As Safe (GRAS) enzymes in both academia and industry, this study reports on an optimized process to achieve polyols transglucosylation using a dextransucrase enzyme derived from Leuconostoc mesenteroides. These enzyme modifications could lead to the creation of a new generation of glucosylated polyols with isomalto-oligosaccharides (IMOS) structures, potentially offering added functionalities such as prebiotic effects. These reactions were guided by a design of experiment framework, aimed at maximizing the yields of potential new sweeteners. Under the optimized conditions, dextransucrase first cleared the glycosidic bond of sucrose, releasing fructose with the formation of an enzyme-glucosyl covalent intermediate complex. Then, the acceptor substrate (i.e., polyols) is bound to the enzyme-glucosyl intermediate, resulting in the transfer of glucosyl unit to the tested polyols. Structural insights into the reaction products were obtained through nuclear maneic resonance (NMR) and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) analyses, which revealed the presence of linear α(1 → 6) glycosidic linkages attached to the polyols, yielding oligosaccharide structures containing from 4 to 10 glucose residues. These new polyols-based oligosaccharides hold promise as innovative prebiotic sweeteners, potentially offering valuable health benefits.

中文翻译：

Leuconostoc mesenteroides Dextransucrase 将转葡萄糖基化异麦芽低聚糖转化为膳食多元醇结构的新型催化方法的设计优化

多元醇或糖醇在工业中广泛用作甜味剂和食品配方成分，旨在对抗与饮食相关的非传染性疾病的发生。鉴于普遍认为安全的（GRAS）酶在学术界和工业界的吸引力，本研究报告了一种使用源自肠系膜明串珠菌的葡糖核酸酶实现多元醇转葡萄糖基化的优化工艺。这些酶修饰可能导致产生具有异麦芽低聚糖（IMOS）结构的新一代葡萄糖基化多元醇，从而可能提供额外的功能，例如益生元效应。这些反应以实验设计框架为指导，旨在最大限度地提高潜在新甜味剂的产量。在优化条件下，右旋糖核酸酶首先清除蔗糖的糖苷键，释放果糖并形成酶-葡萄糖基共价中间复合物。然后，受体底物（即多元醇）与酶-葡萄糖基中间体结合，导致葡萄糖基单元转移到测试的多元醇上。通过核鬃共振（NMR）和基质辅助激光解吸/电离飞行时间（MALDI-TOF）分析获得对反应产物的结构见解，揭示了连接到多元醇的线性 α（1 → 6）糖苷键的存在，产生含有 4 至 10 个葡萄糖残基的寡糖结构。这些基于多元醇的新型低聚糖有望成为创新的益生元甜味剂，可能提供宝贵的健康益处。

更新日期：2024-09-18

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南