Metabolome and Transcriptome Profiling Reveals Age-Associated Variations in Meat Quality and Molecular Mechanisms of Taihe Black-Bone Silky Fowls,Journal of Agricultural and Food Chemistry

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Metabolome and Transcriptome Profiling Reveals Age-Associated Variations in Meat Quality and Molecular Mechanisms of Taihe Black-Bone Silky Fowls
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-18 , DOI: 10.1021/acs.jafc.4c05005
Yunyan Huang ₁ , Chunhui Xu ₁ , Xuan Huang ₁ , Yuting Tan ₁ , Shibao Li ₁ , Zhaozheng Yin ₁

Affiliation

To explore the changes in meat quality and molecular mechanisms during the growth and development of Taihe black-bone silky fowl, this study employed liquid chromatography–mass spectrometry (LC–MS/MS) metabolomics to elucidate the dynamic changes of key differential metabolites (DMs) affecting meat quality, indicating that chicken at D120 had higher levels of ω-3 polyunsaturated fatty acids (PUFAs), creatine, anserine, and homocarnosine, while D150 had the most stachydrine and D210 had the most acylcarnitines. Additionally, D120 and D180 had more umami and sweet compounds. Furthermore, key metabolic pathways influenced by age included purine metabolism, the pentose phosphate pathway, nicotinate and nicotinamide metabolism, and taurine and hypotaurine metabolism. Transcriptomic identified differential expression genes (DEGs) are predominantly enriched in focal adhesion, the TGF-β signaling pathway, and the MAPK signaling pathway. Integrated metabolomics and transcriptomics revealed complex regulatory networks of DEGs and DMs in key metabolic pathways. This research enhanced our understanding of the biology of Taihe black-bone silky fowl meat quality, revealing possible biomarkers.

中文翻译：

代谢组和转录组分析揭示了太和乌骨鸡肉质的年龄相关变化和分子机制

为探究太和乌骨鸡生长发育过程中肉质变化及分子机制，本研究采用液相色谱-质谱（LC-MS/MS）代谢组学技术，阐明关键差异代谢物（DMs）的动态变化。 ) 影响肉质，表明 D120 的鸡肉具有较高水平的 ω-3 多不饱和脂肪酸 (PUFA)、肌酸、鹅氨酸和高肌肽，而 D150 的水苏碱含量最高，D210 的酰基肉碱含量最高。此外，D120 和 D180 具有更多的鲜味和甜味化合物。此外，受年龄影响的关键代谢途径包括嘌呤代谢、磷酸戊糖途径、烟酸盐和烟酰胺代谢以及牛磺酸和亚牛磺酸代谢。转录组鉴定的差异表达基因 (DEG) 主要富集于粘着斑、TGF-β 信号通路和 MAPK 信号通路。综合代谢组学和转录组学揭示了关键代谢途径中 DEG 和 DM 的复杂调控网络。这项研究增强了我们对太和乌鸡肉质生物学的理解，揭示了可能的生物标志物。

更新日期：2024-09-18

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