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Hordenine Alleviates Lipopolysaccharide-Induced Mastitis by Suppressing Inflammation and Oxidative Stress, Modulating Intestinal Microbiota, and Preserving the Blood–Milk Barrier
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-17 , DOI: 10.1021/acs.jafc.4c02867 Yachun Xie 1 , Xinyi Li 1 , Dianwen Xu 1 , Dewei He 2 , Jiaxin Wang 1 , Junlong Bi 3 , Juxiong Liu 1 , Shoupeng Fu 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-17 , DOI: 10.1021/acs.jafc.4c02867 Yachun Xie 1 , Xinyi Li 1 , Dianwen Xu 1 , Dewei He 2 , Jiaxin Wang 1 , Junlong Bi 3 , Juxiong Liu 1 , Shoupeng Fu 1
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Mastitis is a common mammalian disease occurring in the mammary tissue and poses a major threat to agriculture and the dairy industry. Hordenine (HOR), a phenylethylamine alkaloid naturally extracted from malt, has various pharmacological effects, but its role in mastitis is unknown. The aim of this study was to investigate the role of HOR and its underlying mechanism in a lipopolysaccharide (LPS)-induced inflammatory response model of mouse mammary epithelial cells (EpH4-Ev) and mouse mastitis model. The experimental results showed that HOR attenuated LPS-induced mammary tissue damage (from 3.75 ± 0.25 to 1.75 ± 0.25) and restored the integrity of the blood–milk barrier. Further mechanistic studies revealed that HOR inhibited LPS-induced overactivation of the TLR4-MAPK/NF-κB signaling pathway and activated the AMPK/Nrf2/HO-1 signaling pathway. Additionally, HOR altered the composition of the intestinal microbiota in mice, ultimately reducing the extent of inflammatory injury (from 3.33 ± 0.33 to 0.67 ± 0.33) and upregulating the expression of tight junction proteins (ZO-1, occludin, and claudin-3). The findings of this study provide a theoretical basis in the rational use of HOR for the prevention and treatment of mastitis and the maintenance of mammalian mammary gland health.
中文翻译：
大麦芽碱通过抑制炎症和氧化应激、调节肠道微生物群和保护血乳屏障来减轻脂多糖引起的乳腺炎
乳腺炎是一种发生在乳腺组织的常见哺乳动物疾病，对农业和乳品业构成重大威胁。大麦芽碱（HOR）是一种从麦芽中天然提取的苯乙胺生物碱，具有多种药理作用，但其在乳腺炎中的作用尚不清楚。本研究的目的是探讨HOR在脂多糖（LPS）诱导的小鼠乳腺上皮细胞炎症反应模型（EpH4-Ev）和小鼠乳腺炎模型中的作用及其潜在机制。实验结果表明，HOR 减轻了 LPS 引起的乳腺组织损伤（从 3.75 ± 0.25 至 1.75 ± 0.25）并恢复了血乳屏障的完整性。进一步的机制研究表明，HOR 抑制 LPS 诱导的 TLR4-MAPK/NF-κB 信号通路过度激活，并激活 AMPK/Nrf2/HO-1 信号通路。此外，HOR 改变了小鼠肠道微生物群的组成，最终降低了炎症损伤的程度（从 3.33 ± 0.33 到 0.67 ± 0.33）并上调了紧密连接蛋白（ZO-1、occludin 和 claudin-3）的表达。本研究结果为合理使用HOR防治乳腺炎、维护哺乳动物乳腺健康提供理论依据。
更新日期：2024-09-17
中文翻译：
大麦芽碱通过抑制炎症和氧化应激、调节肠道微生物群和保护血乳屏障来减轻脂多糖引起的乳腺炎
乳腺炎是一种发生在乳腺组织的常见哺乳动物疾病，对农业和乳品业构成重大威胁。大麦芽碱（HOR）是一种从麦芽中天然提取的苯乙胺生物碱，具有多种药理作用，但其在乳腺炎中的作用尚不清楚。本研究的目的是探讨HOR在脂多糖（LPS）诱导的小鼠乳腺上皮细胞炎症反应模型（EpH4-Ev）和小鼠乳腺炎模型中的作用及其潜在机制。实验结果表明，HOR 减轻了 LPS 引起的乳腺组织损伤（从 3.75 ± 0.25 至 1.75 ± 0.25）并恢复了血乳屏障的完整性。进一步的机制研究表明，HOR 抑制 LPS 诱导的 TLR4-MAPK/NF-κB 信号通路过度激活，并激活 AMPK/Nrf2/HO-1 信号通路。此外，HOR 改变了小鼠肠道微生物群的组成，最终降低了炎症损伤的程度（从 3.33 ± 0.33 到 0.67 ± 0.33）并上调了紧密连接蛋白（ZO-1、occludin 和 claudin-3）的表达。本研究结果为合理使用HOR防治乳腺炎、维护哺乳动物乳腺健康提供理论依据。
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