In situ characterization techniques of protein corona around nanomaterials,Chemical Society Reviews

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In situ characterization techniques of protein corona around nanomaterials
Chemical Society Reviews ( IF 40.4 ) Pub Date : 2024-09-18 , DOI: 10.1039/d4cs00507d
Fangqin Fu _{1,

2} , Daniel Crespy ₃ , Katharina Landfester ₄ , Shuai Jiang _{1,

2}

Affiliation

Nanoparticles (NPs) inevitably interact with proteins upon exposure to biological fluids, leading to the formation of an adsorption layer known as the “protein corona”. This corona imparts NPs with a new biological identity, directly influencing their interactions with living systems and dictating their fates in vivo. Thus, gaining a comprehensive understanding of the dynamic interplay between NPs and proteins in biological fluids is crucial for predicting therapeutic effects and advancing the clinical translation of nanomedicines. Numerous methods have been established to decode the protein corona fingerprints. However, these methods primarily rely on prior isolation of NP–protein complex from the surrounding medium by centrifugation, resulting in the loss of outer-layer proteins that directly interact with the biological system and determine the in vivo fate of NPs. We discuss here separation techniques as well as in situ characterization methods tailored for comprehensively unraveling the inherent complexities of NP–protein interactions, highlighting the challenges of in situ protein corona characterization and its significance for nanomedicine development and clinical translation.

中文翻译：

纳米材料周围蛋白质电晕的原位表征技术

纳米颗粒（NP）在暴露于生物体液时不可避免地与蛋白质相互作用，导致形成称为“蛋白质电晕”的吸附层。这种电晕赋予 NP 新的生物身份，直接影响它们与生命系统的相互作用并决定它们在体内的命运。因此，全面了解生物体液中 NP 和蛋白质之间的动态相互作用对于预测治疗效果和推进纳米药物的临床转化至关重要。已经建立了许多方法来解码蛋白质电晕指纹图谱。然而，这些方法主要依赖于通过离心从周围培养基中预先分离 NP-蛋白质复合物，导致直接与生物系统相互作用并决定 NP 体内命运的外层蛋白质丢失。我们在这里讨论了分离技术以及为全面揭示 NP-蛋白质相互作用的内在复杂性而量身定制的原位表征方法，强调了原位蛋白质电晕表征的挑战及其对纳米药物开发和临床转化的意义。

更新日期：2024-09-21

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