当前位置: X-MOL 学术Nat. Biotechnol. › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Targeting double-stranded nucleic acids using the λExo–pDNA system
Nature Biotechnology ( IF 33.1 ) Pub Date : 2024-09-18 , DOI: 10.1038/s41587-024-02409-7


λ exonuclease (λExo) binds 5′-phosphorylated single-stranded DNA (pDNA) at complementary regions on double-stranded DNA and DNA–RNA duplexes under ambient conditions without a PAM-like motif. In the presence of Mg2+, λExo then digests the pDNA into nucleotides.

中文翻译:


使用 λExo-pDNA 系统靶向双链核酸



λ 核酸外切酶 (λExo) 在环境条件下在双链 DNA 和 DNA-RNA 双链体的互补区域结合 5'-磷酸化单链 D​​NA (pDNA),无需 PAM 样基序。在 Mg2+ 存在的情况下,λExo 将 pDNA 消化成核苷酸。
更新日期:2024-09-18
down
wechat
bug