In the face of escalating threats posed by human-induced climate change, urgent attention to coral reef restoration is imperative due to ongoing reef degradation. Here, we explored the potential of generating coral micropropagates as a tool to rapidly generate coral tissue for reef restoration and reef engineering. We developed a hypersalinity-induced polyp bailout protocol and a simple attachment device to support the growth of micropropagates on commonly used restoration substrates. We found that hypersalinity induction, at a rate of < 1 ppt hr-1, produced healthy micropropagates of the coral Stylophora pistillata. The highest attachment success (~74%) was achieved in CaCO3 substrate devices, which outperformed PVC (~48%) and Portland cement (~5%). Settled micropropagates displayed rapid growth rates on both CaCO3 (0.037 mm²/day ± 0.002 SE) and PVC (0.057 mm²/day ± 0.008 SE) substrates, while Portland cement induced tissue degradation. Our study provides a detailed methodology for reliably generating, attaching, and growing coral micropropagates and underscores the potential of polyp bailout as a viable technique supporting coral propagation efforts.

中文翻译：

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通过高盐度诱导的珊瑚微繁殖来推进珊瑚繁殖工具包


面对人为气候变化带来的不断升级的威胁，由于珊瑚礁持续退化，迫切需要关注珊瑚礁的恢复。在这里，我们探索了生成珊瑚微繁殖体作为快速生成珊瑚组织以用于珊瑚礁恢复和珊瑚礁工程的工具的潜力。我们开发了一种高盐度诱导的息肉救助方案和一种简单的附着装置，以支持微繁殖体在常用的修复基质上的生长。我们发现，以 < 1 ppt hr-1 的速率进行高盐度诱导，可以产生健康的 Stylophora pistillata 珊瑚微繁殖体。 CaCO3 基底装置实现了最高的附着成功率 (~74%)，其性能优于 PVC (~48%) 和波特兰水泥 (~5%)。沉降的微繁殖体在 CaCO3（0.037 mm²/天 ± 0.002 SE）和 PVC（0.057 mm²/天 ± 0.008 SE）基质上均表现出快速生长速度，而波特兰水泥则诱导组织降解。我们的研究提供了可靠生成、附着和生长珊瑚微繁殖的详细方法，并强调了水螅体救助作为支持珊瑚繁殖工作的可行技术的潜力。