Enhanced inactivation of Aspergillus niger biofilms by the combination of UV-LEDs with chlorine-based disinfectants,Water Research

当前位置： X-MOL 学术 › Water Res. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Enhanced inactivation of Aspergillus niger biofilms by the combination of UV-LEDs with chlorine-based disinfectants
Water Research ( IF 11.4 ) Pub Date : 2024-09-15 , DOI: 10.1016/j.watres.2024.122451
Qiqi Wan ₁ , Jian Ke ₁ , Ruihua Cao ₁ , Jingyi Wang ₁ , Tinglin Huang ₁ , Gang Wen ₂

Affiliation

The presence of pathogenic fungal biofilms in drinking water distribution systems poses significant challenges in maintaining the safety of drinking water. This research delved into the formation of Aspergillus niger (A. niger) biofilms and evaluated their susceptibility to inactivation using combinations of ultraviolet light emitting diodes (UV-LEDs) with chlorine-based disinfectants, including UV-LEDs/chlorine (Cl2), UV-LEDs/chlorine dioxide (ClO2), and UV-LEDs/chloramine (NH2Cl) at 265 nm, 280 nm and 265/280 nm. Results indicated that A. niger biofilms reached initial maturity within 24 h, with matured three-dimensional filamentous structures and conidiospores by 96 h. UV-LEDs combined with chlorine-based disinfectants enhanced A. niger biofilm inactivation compared to UV-LEDs alone and low-pressure UV combined with chlorine-based disinfectants. At an UV fluence of 400 mJ/cm2, log reductions of UV265, UV280, and UV265/280 combined with chlorine-based disinfectants were 2.95-fold, 3.20-fold, and 2.38-fold higher than that of UV265, UV280, and UV265/280, respectively. During the inactivation, A. niger biofilm cells experienced increased membrane permeability and intracellular reactive oxygen species levels, resulting in cellular apoptosis. Extracellular polymeric substances contributed to the higher resistance of biofilms. Regarding electrical energy consumption, the order was: UV-LEDs/ClO2 > UV-LEDs/NH2Cl > UV-LEDs/Cl2. These findings provide insights into the effective utilization of UV-LEDs for fungal biofilm disinfection.

中文翻译：

通过将 UV-LED 与氯基消毒剂相结合，增强黑曲霉生物膜的灭活

饮用水分配系统中病原真菌生物膜的存在对维护饮用水安全构成了重大挑战。这项研究深入研究了黑曲霉（A. niger）生物膜的形成，并使用紫外线发光二极管（UV-LED）与氯基消毒剂的组合评估了它们对灭活的敏感性，包括 UV-LED/氯（Cl2）、UV-LED/二氧化氯（ClO2）和 UV-LED/氯胺（NH2Cl）在 265 nm、280 nm 和 265/280 nm 处。结果表明，黑曲霉生物膜在 24 小时内达到初始成熟，到 96 小时时具有成熟的三维丝状结构和分生孢子。与单独的 UV-LED 和低压紫外线与氯基消毒剂相结合相比，UV-LED 与氯基消毒剂相结合增强了黑曲霉生物膜的灭活。在 400 mJ/cm2 的紫外通量下，UV265、UV280 和 UV265/280 与氯基消毒剂联合使用的对数减少分别比 UV265、UV280 和 UV265/280 高 2.95 倍、3.20 倍和 2.38 倍。在灭活过程中，A. niger 生物膜细胞的膜通透性和细胞内活性氧水平增加，导致细胞凋亡。细胞外聚合物物质有助于生物膜的更高抵抗力。关于电能消耗，顺序为： UV-LEDs/ClO2 > UV-LED/NH2Cl > UV-LED/Cl2。这些发现为有效利用 UV-LED 进行真菌生物膜消毒提供了见解。

更新日期：2024-09-15

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南