This study discussed the role of plant-associated microbiome in regulating ARG transfer in soil-plant systems. Results showed that target ARGs in plants were mainly derived from rhizosphere soil. Cooperative interactions among bacteria in rhizosphere soil, plant-roots, plant-shoots, and soil-roots-shoots systems occurred during ARG transfer. The number of modules and keystone taxa identified as positively correlated with ARG transfer in rhizosphere soil, roots, and shoots was 3 and 49, 3 and 41, 2 and 5, respectively. Among these modules, module 3 in roots was significantly positively correlated with module 3 in rhizosphere soils and module 2 in shoots, indicating that module 3 in roots played central hub roles in ARG transfer from rhizosphere soil to roost and shoots. This may be because module 3 in roots increased cell motility and xenobiotics biodegradation and metabolism. These keystone taxa mainly belonged to Proteobacteria that can carry ARGs to transfer in soil-plant systems, especially Clostridium-sensu_stricito and Pseudomonas in rhizosphere soil carried ARGs into the shoot. Additionally, they promoted ARG transfer by increasing plant biomass, net photosynthetic rate and water use efficiency. The findings helped reveal the mechanism of plant-associated bacterial interactions and provided understanding for potential risks of ARG transfer from soil to plants.

中文翻译：

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深入了解植物相关细菌的相互作用及其在抗生素抗性基因从土壤转移到植物中的作用


本研究讨论了植物相关微生物组在调节土壤-植物系统中 ARG 转移中的作用。结果表明，植物中的目标 ARGs 主要来源于根际土壤。在 ARG 转移过程中，根际土壤、植物根、植物芽和土壤根芽系统中的细菌之间发生了合作相互作用。与根际土壤、根和芽中的 ARG 转移呈正相关的模块和关键分类群的数量分别为 3 和 49、3 和 41、2 和 5。在这些模块中，根中的模块 3 与根际土壤中的模块 3 和芽中的模块 2 呈显著正相关，表明根中的模块 3 在 ARG 从根际土壤向栖息地和芽的转移中起中心枢纽作用。这可能是因为根中的模块 3 增加了细胞运动和外源性生物降解和新陈代谢。这些关键类群主要属于能携带ARGs在土壤-植物系统中转移的变形菌门，尤其是根际土壤中的梭菌属（Clostridium-sensu_stricito Pseudomonas）携带ARGs进入地上部。此外，他们通过增加植物生物量、净光合速率和水利用效率来促进 ARG 转移。这些发现有助于揭示植物相关细菌相互作用的机制，并为 ARG 从土壤转移到植物的潜在风险提供了理解。