SummaryMicroRNA827 (miR827) is functionally conserved among different plant species and displays species‐specific characteristics, but the mechanisms by which miR827 regulates phosphate (Pi) starvation tolerance and maize development remain elusive. We found that miR827 selectively targets the Pi transporter genes SPX‐MFS1 and SPX‐MFS5. miR827 overexpression improved the Pi starvation tolerance, plant architecture and grain yield and quality, whereas miR827 suppression yielded a contrasting phenotype. In addition, we identified a specific long noncoding RNA (lncRNA767) that serves as a direct target and a facilitator of miR827 and can stabilize the SPX‐MFS1 and SPX‐MFS5 transcripts, leading to their translation inhibition. The orchestrated regulation of SPX‐MFS1 and SPX‐MFS5 modulates PHR1; 1 and PHR1; 2, which are critical transcription factors in Pi signalling, and thereby affects the expression of downstream Pi starvation‐induced genes. Together, these findings demonstrate that miR827, assisted by lncRNA767, enhances SPX‐MFS1 and SPX‐MFS5 suppression and thus exerts a significant impact on Pi homeostasis and several essential agronomic traits of maize.

中文翻译：

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miR827在lncRNA767的协助下协调SPX-MFS1和SPX-MFS5的调节，以增强磷酸盐饥饿耐受性和玉米发育


摘要MicroRNA827 (miR827) 在不同植物物种中功能保守，并表现出物种特异性特征，但 miR827 调节磷酸盐 (Pi) 饥饿耐受性和玉米发育的机制仍然难以捉摸。我们发现 miR827 选择性靶向 Pi 转运蛋白基因 SPX-MFS1 和 SPX-MFS5。 miR827 过表达改善了 Pi 饥饿耐受性、植物结构以及谷物产量和质量，而 miR827 抑制则产生了相反的表型。此外，我们还发现了一种特定的长非编码RNA（lncRNA767），它作为miR827的直接靶标和促进子，可以稳定SPX-MFS1和SPX-MFS5转录本，从而抑制它们的翻译。 SPX-MFS1 和 SPX-MFS5 的协调调控可调节 PHR1； 1和PHR1； 2，它们是 Pi 信号转导中的关键转录因子，从而影响下游 Pi 饥饿诱导基因的表达。总之，这些发现表明，miR827 在 lncRNA767 的协助下，增强了 SPX-MFS1 和 SPX-MFS5 的抑制，从而对 Pi 稳态和玉米的几个重要农艺性状产生重大影响。