A novel and selective (deep eutectic solvent) DES-based microextraction method was established for the first time, utilizing a synthesized new generation High-Density Type-V DES for monitoring the dye Erythrosine (E127) in various foodstuffs and drugs. Type-V DES was created from acetophenone and diphenylamine at 3:1 M ratio. The pH, DES amount, and vortex time were optimized using Box-Behnken Design (BBD) of Response Surface Methodology (RSM). The quadratic microextraction model with R2 = 0.9982 was obtained. The limit of detection, preconcentration factor and linear dynamic range were determined to be 12 μg/L, 50 and 41–4000 μg/L, respectively. Effects of matrix components were examined. The developed High-Density Type-V Deep Eutectic Solvent Microextraction (HD-V-DES-ME) method was applied to foodstuffs and drugs to monitor their E127 contents and subsequently validated by applying spiked tests to real samples, with recoveries ranging between 94 and 101 %. The indexes of environmental friendliness and practicality for the method were evaluated using the Analytical GREEnness metric approach tool (AGREE) and the Blue Applicability Grade Index tool (BAGI), respectively.

中文翻译：

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使用新一代高密度 V 型深共熔溶剂选择性微萃取食品中的赤藓红 （E127）


首次建立了一种新颖的选择性（低共熔溶剂）基于 DES 的微萃取方法，利用合成的新一代高密度 V 型 DES 来监测各种食品和药物中的染料赤藓红 （E127）。V 型 DES 由苯乙酮和二苯胺以 3：1 M 的比例制成。使用响应面法 （RSM） 的 Box-Behnken 设计 （BBD） 优化 pH 、 DES 量和涡旋时间。获得了 R2 = 0.9982 的二次微萃取模型。检测限、预浓缩因子和线性动态范围分别为 12 μg/L、50 和 41–4000 μg/L。检查了基质成分的影响。开发的高密度 V 型深共晶溶剂微萃取 （HD-V-DES-ME） 方法应用于食品和药品，以监测其 E127 含量，随后通过对实际样品进行加标测试进行验证，回收率在 94% 到 101% 之间。分别使用分析 GREEnness 度量方法工具 （AGREE） 和蓝色适用性等级指数工具 （BAGI） 评估该方法的环境友好性和实用性指标。