Allergy to novel food proteins, due to diverse ingredients and innovative food processing technologies employed to achieve desired functional properties, is a major safety concern. Current allergy testing methods (ELISA and mass spectrometry) depend on high-quality protein extracts, meaning existing methods are often tailored to specific matrices. Therefore, a more efficient and general protein extraction method is desirable for comprehensive allergy risk assessment. Here, we developed a highly efficient and reproducible protein extraction method which achieved at least 80 % efficiency across several food matrices. Proteomics analysis of a plant-based meat using our optimized extraction method showed that higher extraction efficiency improved reproducibility of identified proteins. Moreover, higher protein extraction efficiency resulted in increased abundances of individual allergenic proteins. This underscores the relevance of our method for more accurate measurements of allergenic protein concentrations in allergy risk assessments.

中文翻译：

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用于评估复杂替代蛋白质基质中食物过敏风险的高效蛋白质提取


由于采用多种成分和创新的食品加工技术来实现所需的功能特性，对新型食品蛋白质过敏是一个主要的安全问题。目前的过敏检测方法（ELISA 和质谱法）依赖于高质量的蛋白质提取物，这意味着现有方法通常是针对特定基质量身定制的。因此，对于全面的过敏风险评估，需要一种更有效和通用的蛋白质提取方法。在这里，我们开发了一种高效且可重现的蛋白质提取方法，该方法在多种食品基质中实现了至少 80% 的效率。使用我们优化的提取方法对植物肉进行的蛋白质组学分析表明，更高的提取效率提高了鉴定蛋白质的可重复性。此外，更高的蛋白质提取效率导致单个过敏蛋白的丰度增加。这强调了我们的方法在过敏风险评估中更准确地测量过敏原蛋白浓度的相关性。