Acute lymphoblastic leukaemia (ALL) is a complex disease in pediatric oncology, necessitating accurate diagnostic strategies for effective treatment planning. The ability to differentiate between B-cell ALL (B-ALL) and T-cell ALL (T-ALL) is crucial for targeted interventions. However, current diagnostic methods are time-consuming and require rapid, dependable tests. This study explores the potential of label-free Raman imaging coupled with chemometrics for rapid blast phenotyping of B-ALL and T-ALL. Our findings demonstrate the efficacy of Raman spectroscopy in sensitively and specifically screening and classifying ALL, as well as its rapidity and reliability. The obtained molecular information allows for label-free and precise leukaemia diagnosis at the single-cell level, surpassing the capabilities of traditional diagnostic techniques. Raman spectra of cancer cells reveal distinctive molecular signatures, specifically heightened protein and nucleic acid content, revealing molecular signatures unique to leukemic phenotypes. Based on that, they could be distinguished from each other and their normal B and T lymphocyte counterparts. This research underscores the analytical power of Raman spectroscopy, positioning it as a valuable tool for identifying and classifying pediatric ALL subtypes. The potential translational applications in clinical practice offer a promising avenue for an expedited and accurate leukaemia diagnosis, paving the way for more targeted and personalised therapeutic approaches.

中文翻译：

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推进急性淋巴细胞白血病亚型诊断的分类：用于精确单细胞表型和亚型分类的无标记拉曼光谱


急性淋巴细胞白血病 （ALL） 是儿科肿瘤学中的一种复杂疾病，需要准确的诊断策略才能制定有效的治疗计划。区分 B 细胞 ALL （B-ALL） 和 T 细胞 ALL （T-ALL） 的能力对于有针对性的干预至关重要。然而，目前的诊断方法非常耗时，并且需要快速、可靠的测试。本研究探讨了无标记拉曼成像结合化学计量学对 B-ALL 和 T-ALL 进行快速原始细胞表型分析的潜力。我们的研究结果表明，拉曼光谱在灵敏、特异性地筛查和分类 ALL 方面的有效性，以及其快速性和可靠性。获得的分子信息允许在单细胞水平上进行无标记和精确的白血病诊断，超越了传统诊断技术的能力。癌细胞的拉曼光谱揭示了独特的分子特征，特别是蛋白质和核酸含量的增加，揭示了白血病表型特有的分子特征。基于此，可以区分它们彼此以及正常的 B 和 T 淋巴细胞对应物。这项研究强调了拉曼光谱的分析能力，将其定位为识别和分类儿科 ALL 亚型的宝贵工具。临床实践中的潜在转化应用为快速和准确的白血病诊断提供了一条有前途的途径，为更具针对性和个性化的治疗方法铺平了道路。