Engineered hydrogel patches have shown promising therapeutic effects in the treatment of myocardial infarction (MI), especially anisotropic patches that mimic the characteristics of native myocardium have attracted widespread attention. However, it remains a great challenge to develop cardiac patches with long-range and orderly electrical conduction based on an effective, mild, and rapid strategy. Here, a multifunctional anisotropic cardiac patch is presented based on microfluidic manipulation. The anisotropic alginate-gelatin methacrylate hydrogel patches are easily and rapidly prepared through microfluidic focusing, ion-photocrosslinking, and parallel packing processes. The fluid-based anisotropic realization process does not involve complex machining and strong field stimulation and is compatible with the loading of macromolecular biological agents. The anisotropic hydrogel patch can mimic the anisotropy of the myocardium and guide the directional polarization of cardiomyocytes. In animal model experiments, it also exhibits significant effects in inhibiting ventricular remodeling, fibrosis, and enhancing cardiac function recovery after MI. These comprehensive features make the multifunctional hydrogel patch a promising candidate for cardiac tissue repair and future provide a new paradigm for expanding microfluidic technology to solve tissue engineering challenges.

中文翻译：

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一种通过微流控操作制造的多功能各向异性贴剂，用于修复梗死心肌


工程水凝胶贴剂在治疗心肌梗死 （MI） 方面显示出良好的治疗效果，尤其是模仿天然心肌特性的各向异性贴剂引起了广泛关注。然而，基于有效、温和和快速的策略开发具有长距离有序电传导的心脏补片仍然是一个巨大的挑战。在这里，提出了一种基于微流体操作的多功能各向异性心脏补片。各向异性藻酸盐-明胶甲基丙烯酸酯水凝胶贴剂可通过微流体聚焦、离子-光交联和平行填充工艺轻松快速地制备。基于流体的各向异性实现过程不涉及复杂的加工和强场刺激，并且与大分子生物制剂的负载兼容。各向异性水凝胶贴片可以模拟心肌的各向异性并引导心肌细胞的定向极化。在动物模型实验中，它在抑制心肌梗死后心室重塑、纤维化和增强心功能恢复方面也表现出显著效果。这些全面的特性使多功能水凝胶贴剂成为心脏组织修复的有前途的候选者，未来为扩展微流控技术以解决组织工程挑战提供了新的范式。