In the present study, silver (Ag), magnesium (Mg), and the various molar ratios of Ag:Mg-doped copper oxide nanocomposites (CuO NCs) were synthesized by the co-precipitation method. The CuO NC samples calcined at 500 °C were further analyzed for their morphological, structural, functional, and optical properties. Adding single and dual doping increased the pristine CuO band gap from 1.28 to 1.50 eV. The Ag and Mg metal peaks were revealed in the Fourier transform infrared (FTIR) analysis, while an elevated Mg band was observed in all Mg-doped samples in the Raman results. All CuO NCs showed a monoclinic crystalline structure, flake, and rod-like morphologies. Microbes of Bacillus subtilis, Shigella dysenteriae, and Candida albicans were tested against different concentrations of CuO NCs. Better results for all tested microbes were observed with the 2 mg concentration. The high cytotoxicity effect was observed in the pristine and Cu:Ag (94:6) sample, and other CuO NCs, which demonstrated over 80 % viability in RAW 246.7 cells at a concentration of 0.5 µg/ml. Remarkably, over 94 % cell viability at 0.5 µg/ml was achieved with the Cu:Ag:Mg (94:3:3) NCs ratio, owing to the synergistic effect of the ion-releasing ability of Cu2+, Ag2+, and Mg2+ ions. It possesses a distinctive high aspect ratio shape, is ion-releasing, and has excellent cytocompatibility. The suitability of the Cu:Ag:Mg (94:3:3) NCs ratio for addressing microbial challenges in healthcare is suggested by their distinct characteristics, indicating the promising potential for future biomedical applications.

中文翻译：

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通过氧化铜纳米复合材料的银和镁掺杂策略增强抗菌性能和细胞相容性


在本研究中，采用共沉淀法合成了银（Ag）、镁（Mg）以及不同摩尔比的Ag:Mg掺杂氧化铜纳米复合材料（CuO NCs）。进一步分析了在 500 °C 下煅烧的 CuO NC 样品的形态、结构、功能和光学特性。添加单掺杂和双掺杂将原始 CuO 带隙从 1.28 eV 增加到 1.50 eV。傅里叶变换红外 (FTIR) 分析显示了 Ag 和 Mg 金属峰，而拉曼结果中所有掺镁样品中都观察到了升高的 Mg 带。所有 CuO NCs 均呈现单斜晶体结构、片状和棒状形态。针对不同浓度的 CuO NCs 测试了枯草芽孢杆菌、痢疾志贺氏菌和白色念珠菌的微生物。在 2 mg 浓度下，所有测试微生物均获得了更好的结果。在原始样品和 Cu:Ag (94:6) 样品以及其他 CuO NC 中观察到高细胞毒性作用，在浓度为 0.5 µg/ml 的 RAW 246.7 细胞中显示出超过 80% 的活力。值得注意的是，由于 Cu2+、Ag2+ 和 Mg2+ 离子释放能力的协同作用，Cu:Ag:Mg (94:3:3) NCs 比例在 0.5 µg/ml 条件下实现了超过 94% 的细胞活力。它具有独特的高纵横比形状，可释放离子，并具有优异的细胞相容性。 Cu:Ag:Mg (94:3:3) NCs 比例的独特特性表明其适合解决医疗保健中的微生物挑战，表明未来生物医学应用的巨大潜力。