S-nitrosoglutathione (GSNO) is the most important S-nitrosothiol in vivo, which could affect the quality of meat by participating in calcium release, glucose metabolism, proteolysis and apoptosis, therefore may potentially serve as a marker for meat freshness. In this work, a solid-phase extraction (SPE) monolithic spin column modified with gold nanoparticles was prepared for GSNO extraction. The optimized SPE-LC-MS/MS method for GSNO quantification displays low limit of detection (0.01 nM), good precision (RSD < 15 %) and acceptable recovery (> 77.7 %). Furthermore, this approach has been applied to monitor GSNO levels in beef and pork stored at −20 °C for different days, showing that endogenous GSNO level increases during prolonged storage and could be employed as a marker to evaluate the freshness of ice stored meat. Additionally, the monolithic spin column remains in good quality after a half-year storage, which is promising to develop into commercial enrichment kit for endogenous GSNO analysis.

中文翻译：

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金纳米颗粒功能化聚合物整体在离心柱中测定肉类中 S-亚硝基谷胱甘肽的制备及应用


S-亚硝基谷胱甘肽 （GSNO） 是体内最重要的 S-亚硝基硫醇，可通过参与钙释放、葡萄糖代谢、蛋白水解和细胞凋亡来影响肉类品质，因此可能作为肉类新鲜度的标志物。在本工作中，制备了一种用金纳米颗粒修饰的固相萃取 （SPE） 整体离心柱，用于 GSNO 萃取。优化的 SPE-LC-MS/MS 方法用于 GSNO 定量，检测限低 （0.01 nM）、精密度好 （RSD < 15 %） 和可接受的回收率 （> 77.7 %）。此外，该方法已被应用于监测在 -20 °C 下储存不同天数的牛肉和猪肉中的 GSNO 水平，表明内源性 GSNO 水平在长期储存过程中会增加，并可用作评估冰储存肉类新鲜度的标志物。此外，整体离心柱在储存半年后仍保持良好的质量，有望开发成用于内源性 GSNO 分析的商业富集试剂盒。