It has been reported that eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in glycerides have various biological functions. This study presents an effective method for enriching glycerides rich in EPA and DHA through lipase-catalyzed alcoholysis. The results showed that Eversa® Transform 2.0 had the strongest discrimination against DHA and EPA in alcoholysis, which was verified by molecular docking. Additionally, selectivity of the lipase and ratio of DHA and EPA in glyceride products were significantly affected by alcohol type. Under the optimal conditions, the contents of EPA and DHA in glycerides after ethanolysis reached 12.91 % and 55.40 %, respectively, with a DHA yield of 79.22 %. In this study, an interesting finding was that Eversa® Transform 2.0 could effectively differentiate EPA and DHA during alcoholysis to allow us to prepare DHA-enriched glycerides and EPA-enriched ethyl esters after removing saturated and monounsaturated ethyl esters.

中文翻译：

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通过选择性酶促乙醇解法富集甘油酯中的 EPA 和 DHA


据报道，甘油酯中的二十碳五烯酸 （EPA） 和二十二碳六烯酸 （DHA） 具有多种生物学功能。本研究提出了一种通过脂肪酶催化的醇解来富集富含 EPA 和 DHA 的甘油酯的有效方法。结果表明，Eversa® Transform 2.0 对醇解 DHA 和 EPA 的区分力最强，通过分子对接验证。此外，甘油酯产品中脂肪酶的选择性以及 DHA 和 EPA 的比例受酒精类型的显著影响。在最佳条件下，乙醇解冻后甘油酯中EPA和DHA的含量分别达到12.91%和55.40 %，DHA得率为79.22 %。在这项研究中，一个有趣的发现是 Eversa® Transform 2.0 可以在醇解过程中有效区分 EPA 和 DHA，使我们能够在去除饱和和单不饱和乙酯后制备富含 DHA 的甘油酯和富含 EPA 的乙酯。