There is increasing interest in using locally sourced soybeans and soybean meal. Ileal amino acid digestibility (IAAD) of soybean meal from Kentucky-grown soybeans was evaluated in 25- and 50-kg cannulated pigs. The experiment used sixteen pigs in a randomized complete block design consisting of four treatments with 4 pigs per treatment. This was repeated in time resulting in eight replicates per treatment for both body weight (BW)groups. After cannulation at about 20 kg BW, all pigs were allowed to recover from the surgery and were fed a corn-SBM-based diet that met all nutrient and energy requirements. The experimental diets consisted of two nitrogen-free diets [NFD] with or without exogenous enzyme supplementation (phytase and a combination of phytase and carbohydrase enzymes) and two semi-purified diets containing soybean meal from Kentucky-grown soybeans as the only source of protein with or without exogenous enzyme supplementation. Each diet was fed for 7 consecutive d with 10-h ileal digesta collection on d 6 and 7. The same diets were fed to the same set of pigs at about 50 kg BW. Data were analyzed as a factorial arrangement of treatments (BW x diet) for ileal endogenous amino acid losses (IEAAL), apparent IAAD and standardized IAAD (SIAAD). Data from the NFD were used to determine the SIAAD from the AIAAD values. Exogenous enzyme supplementation increased (P < 0.05) IEAAL except for Gly and Pro while samples collected during phase 2 had greater (P < 0.05) IEAAL (10 amino acids). Pig BW did not influence IEAAL. Enzyme supplementation did not increase AIAAD except for Arg, Ala, Gly, N, and total AA while apparent ileal N, Met, Thr, and Val digestibility was greater (P < 0.05) in 50 kg pigs. Interactions between BW and diets showed that 50 kg pigs fed the SBM diet containing enzyme supplementation had greater (P < 0.05) N, Arg, and total AA digestibility compared with 50 kg pigs without enzyme supplementation. Digestibility of Val and Asp was greater (P < 0.05) in samples collected during phase 2. After correction for basal IEAAL, 50 kg pigs had greater (P < 0.05) SIAAD values for 12 of the AA analyzed while exogenous enzyme supplementation resulted in greater (P < 0.05) SIAAD for 9 of the AA analyzed. The SIAAD values for four AA (His, Phe, Val, and Asp) were greater (P < 0.05) for samples collected in phase 2. Results from this study showed that exogenous enzyme supplementation increased IEAAL and that IAAD of SBM produced from Kentucky-cultivated soybeans was high and that exogenous enzyme supplementation improved the SIAAD values of several amino acids. The IAAD values of SBM produced from soybeans cultivated in KY was high and are similar to values reported in the literature.

中文翻译：

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PSVI-29 猪体重和外源酶补充对肯塔基州种植的大豆粕的回肠内源氨基酸损失和标准化回肠氨基酸消化率的影响


人们对使用当地采购的大豆和豆粕的兴趣越来越大。在 25 公斤和 50 公斤的罐头猪中评估了肯塔基州种植的大豆豆粕的回肠氨基酸消化率 （IAAD）。该试验在随机完全区组设计中使用了 16 头猪，该设计由 4 个处理组成，每个处理 4 头猪。及时重复此操作，导致两个体重 （BW） 组每次处理 8 次重复。在约 20 kg 体重插管后，所有猪都从手术中恢复，并饲喂满足所有营养和能量需求的基于玉米 SBM 的日粮。实验日粮包括两种无氮日粮 [NFD]，有或没有外源性酶补充剂（植酸酶和植酸酶和碳水化合物酶的组合）和两种半纯化日粮，其中含有来自肯塔基州种植的大豆的豆粕作为唯一的蛋白质来源，有或没有外源性酶补充剂。每种饮食连续饲喂 7 天，并在第 6 天和第 7 天收集 10 小时的回肠消化物。将相同的日粮饲喂给同一组猪，体重约为 50 公斤。数据作为回肠内源性氨基酸丢失 （IEAAL） 的析因治疗安排 （BW x 饮食） 进行分析，表观 IAAD 和标准化 IAAD （SIAAD）。来自 NFD 的数据用于从 AIAAD 值中确定 SIAAD。外源酶添加增加 （P < 0.05） IEAAL 除 Gly 和 Pro 外，而第 2 阶段收集的样品具有更多 （P < 0.05） IEAAL （10 个氨基酸）。Pig BW 对 IEAAL 没有影响。除 Arg、Ala、Gly、N 和总 AA 外，酶补充剂没有增加 AIAAD，而 50 kg 猪的表观回肠 N、Met、Thr 和 Val 消化率更高 （P < 0.05）。 BW 与日粮之间的交互作用表明，与未补充酶的 50 kg 猪相比，饲喂含有酶补充剂的 SBM 日粮的 50 kg 猪具有更高的 （P < 0.05） N、Arg 和总 AA 消化率。在第 2 阶段收集的样品中，Val 和 Asp 的消化率较高 （P < 0.05）。校正基础 IEAAL 后，50 kg 猪在 12 种分析的 AA 中具有更高的 （P < 0.05） SIAAD 值，而外源酶补充剂导致 9 种 AA 的 SIAAD 更高 （P < 0.05）。在第 2 阶段收集的样品中，四种 AA （His、Phe、Val 和 Asp） 的 SIAAD 值更高 （P < 0.05）。这项研究的结果表明，外源酶补充剂增加了 IEAAL并且肯塔基州种植的大豆产生的 SBM 的 IAAD 很高，并且外源酶补充剂提高了几种氨基酸的 SIAAD 值。由肯塔基州种植的大豆生产的 SBM 的 IAAD 值很高，与文献中报道的值相似。