The objective of this study was to evaluate the effects of supplementation of vitamin B complex on post-weaning performance of beef calves. The study was conducted during fall of 2023 at Eastern Oregon Agriculture Research Center (EOARC; Burns, OR). At weaning (d 0), 63 calves [Angus × Hereford; body weight (BW) = 252 ± 3.6 kg] were randomly assigned to 1 of 3 treatments: 1) Control, 2) VitB1, or 3) VitB2. Calves assigned to Control treatment served as a negative control and were not supplemented with vitamin B complex. Calves assigned to treatment VitB1 were supplemented at 1g/calf daily, while calves assigned to VitB2 treatment were supplemented at 2g/calf daily of vitamin B complex during the 42 d of preconditioning. Upon weaning processing, calves were allocated to 21 pens (3 calves/pen) and treatments were allocated to each pen (7 pens/treatment). Vitamin B complex [Vivalto, pantothenic acid (B5; 40,000 mg/kg), pyridoxine (B6; 20,000 mg/kg), folic acid (B9; 3,200 mg/kg), biotin (B7; 3,200,000 mcg/kg), cobalamin (B12; 320,000 mcg/kg); Selko USA, Indianapolis, IN] supplementation was offered at pen level beginning on d 1 and until the end of the preconditioning phase (d 42). Vitamin B complex was offered to calves assigned to vitamin B complex treatment mixed with 1.3 kg of dried distiller’s grains. Additionally, calves had free access to chopped alfalfa-grass hay mix and whole corn. Pen intake was recorded daily. On d 0, BW and blood samples were collected from all calves. Additional blood and BW were collected on d 1, 3, 7, 14, 21, 42 post-weaning. Body weights were used to calculate calf average daily gain (ADG) and blood samples were assayed for acute phase proteins (ceruloplasmin and haptoglobin). Additional blood samples will be analyzed for cortisol and vitamin B concentrations. Variables were analyzed considering pen as the experimental unit and pen(treatment) as a random effect. Blood variables were evaluated as repeated measures and effects of treatment, day, and treatment the interactions were analyzed with the MIXED procedure of SAS (SAS Inst. Inc., Cary, NC). Significance was set at P ≤ 0.05. No treatment effects (P = 0.98) were observed for final BW (283, 283, and 284 kg respectively for Control, Vit1, and Vit2) or for ADG (P = 0.53; 0.735, 0.795, and 0.747 kg/d respectively for Control, Vit1, and Vit2). Similarly, no treatment effects were observed for the acute phase proteins analyzed, ceruloplasmin (P = 0.66; 27, 30, and 33 µg/dL respectively for Control, Vit1, and Vit2) and haptoglobin (P = 0.41; 0.55, 0.62, and 0.59 mg/mL, respectively for Control, Vit1, and Vit2). Supplementation of vitamin B complex to beef calves post-weaning does not seem to affect calf performance, regardless of the level of supplementation.

中文翻译：

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363 补充维生素 B 复合物对 42 天预处理程序期间肉犊生产性能的影响


本研究的目的是评估补充维生素 B 复合物对肉牛犊断奶后生产性能的影响。该研究于 2023 年秋季在俄勒冈州东部农业研究中心 （EOARC;伯恩斯，俄勒冈州）。断奶时 （d 0），将 63 头犊牛 [Angus × Hereford;体重 （BW） = 252 ± 3.6 kg] 随机分配到 3 种处理中的 1 种：1） 对照，2） VitB1，或 3） VitB2。分配到对照治疗的犊牛作为阴性对照，未补充维生素 B 复合物。在预处理的 42 d 期间，分配到 VitB1 处理组的犊牛每天补充 1 g/犊牛，而分配到 VitB2 处理组的犊牛每天补充 2 g/犊牛的维生素 B 复合物。在断奶处理时，将犊牛分配到 21 个猪栏（3 个犊牛/栏），并将治疗分配给每个猪栏（7 个猪栏/处理）。维生素 B 复合物 [Vivalto、泛酸 （B5;40,000 mg/kg）、吡哆醇 （B6;20,000 mg/kg）、叶酸 （B9;3,200 mg/kg）、生物素 （B7;3,200,000 mcg/kg）、钴胺素 （B12;320,000 mcg/kg）;Selko USA，印第安纳波利斯，印第安纳州] 从第 1 天开始在笔水平提供补充剂，直到预处理阶段结束 （d 42）。向分配到维生素 B 复合物治疗的犊牛提供维生素 B 复合物，与 1.3 公斤干酒糟混合。此外，犊牛可以免费获得切碎的苜蓿草干草混合物和整粒玉米。每天记录笔摄入量。第 0 天，从所有犊牛收集 BW 和血液样本。在断奶后第 1 天、第 3 天、第 7 天、第 14 天、第 21 天、第 42 天收集额外的血液和 BW。体重用于计算犊牛平均日增重 （ADG），并检测血样中的急性期蛋白 （铜蓝蛋白和结合珠蛋白）。 将分析额外的血液样本的皮质醇和维生素 B 浓度。分析变量时，将 pen 作为实验单位，pen（treatment） 作为随机效应。血液变量作为重复测量进行评估，并使用 SAS 的 MIXED 程序 （SAS Inst. Inc.， Cary， NC） 分析治疗、日期和治疗效果的相互作用。显著性设置为 P ≤ 0.05。最终 BW （对照、Vit1 和 Vit2 分别为 283、283 和 284 kg） 或 ADG （P = 0.53;对照、Vit1 和 Vit2 分别为 0.735、0.795 和 0.747 kg/d）未观察到治疗效果 （P = 0.98）。同样，未观察到对分析的急性期蛋白、铜蓝蛋白（P = 0.66;对照、Vit1 和 Vit2 分别为 27、30 和 33 μg/dL）和结合珠蛋白（P = 0.41;对照、Vit1 和 Vit2 分别为 0.55、0.62 和 0.59 mg/mL）的治疗效果。断奶后为肉牛补充维生素 B 复合物似乎不会影响犊牛的生产性能，无论补充水平如何。