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Dietary supplementation with succinic acid improves growth performance and flesh quality of adult Nile tilapia (Oreochromis niloticus) fed a high-carbohydrate diet
Animal Nutrition ( IF 6.1 ) Pub Date : 2024-05-25 , DOI: 10.1016/j.aninu.2024.04.016
Manxia Cao , Ningning Xie , Jianmin Zhang , Ming Jiang , Feng Huang , Lixue Dong , Xing Lu , Hua Wen , Juan Tian

To evaluate the effects of dietary supplementation with succinic acid on growth performance, flesh quality, glucose, and lipid metabolism of Nile tilapia (Oreochromis niloticus) fed a high-carbohydrate diet (HCD), five iso-nitrogenous and iso-lipidic diets were prepared as follows: HCD (control group) consisting of 55% corn starch and HCD supplemented with 0.5%, 1.0%, 2.0%, and 4.0% succinic acid, respectively. Tilapia with an initial body weight of 204.90 ± 1.23 g randomly assigned to 15 tanks with 3 replicates per group and 10 fish per tank fed for 8 weeks. Increasing dietary succinic acid supplementation resulted in significant second-order polynomial relationship in the weight gain rate (WGR), specific growth rate (SGR), feed conversion ratio (FCR), protein efficiency rate (PER), viscerosomatic index, condition factor, and contents of muscular crude lipid and glycogen (P < 0.05). The hepatosomatic index, mesenteric fat index, liver glycogen content and crude lipid contents of the whole-body and liver demonstrated significantly linear and second-order polynomial relationship (P < 0.05). Quadratic curve model analysis based on WGR, SGR, PER, and FCR demonstrated that optimal supplementation with succinic acid in the HCD of Nile tilapia ranged from 1.83% to 2.43%. Fish fed with 1.0% succinic acid had higher muscular hardness, increased the contents of alkali-soluble hydroxyproline in collagen, docosahexaenoic acid (DHA) and n-3 polyunsaturated fatty acid (n-3PUFA) in muscle, and lower total fatty acid content in muscle (P < 0.05) compared with the control group. Compared to the control group, dietary supplementation with 1.0% succinic acid significantly increased the contents of total bounding amino acid (arginine, histidine, isoleucine, lysine, methionine, alanine, proline), total flavor amino acid (free aspartic acid), the catalase (CAT) activity and total antioxidant capacity, and the mRNA relative expression levels of CAT, superoxide dismutase (SOD), and nuclearfactor erythroidderived 2-like 2 (Nrf2) in muscle (P < 0.05). Furthermore, succinic acid supplementation significantly up-regulated mRNA relative expression levels of glycolysis genes (hexokinase 2 [HK2], phosphofructokinase, muscle-A [PFKMA], and phosphofructokinase, muscle-B [PFKMB]), a key glycogen synthesis gene (glycogen synthase [GYS]), and lipid catabolism genes (carnitine palmitoyltransferase-1B [CPT1B], hormone sensitive lipase [HSL], and lipoprotein lipase [LPL]), while down-regulating the mRNA relative expression level of fatty acid synthase (FASN) in muscle (P < 0.05). In conclusion, dietary supplementation with 1.83% to 2.43% succinic acid improved muscle quality by increasing muscle antioxidant capacity and hardness, changing muscle amino acid and fatty acid composition, and regulating muscle glucose and lipid metabolism.

中文翻译:


膳食补充琥珀酸可改善饲喂高碳水化合物饮食的成年尼罗罗非鱼 (Oreochromis niloticus) 的生长性能和肉质



为评价日粮中添加琥珀酸对饲喂高碳水化合物日粮 (HCD) 的尼罗罗非鱼 (Oreochromis niloticus) 生长性能、果肉品质、葡萄糖和脂质代谢的影响,制备了以下 5 种异氮和等脂日粮:由 55% 玉米淀粉和 HCD 添加 0.5%、1.0%、2.0% 和 4.0% 琥珀酸的 HCD 组成, 分别。初始体重为 204.90 ± 1.23 克的罗非鱼随机分配到 15 个水箱,每组 3 次重复,每个水箱 10 条鱼,喂食 8 周。增加日粮中添加丁二酸导致增重率 (WGR) 、比生长率 (SGR) 、饲料转化率 (FCR) 、蛋白质效率 (PER) 、内脏血清学指数、条件因子以及肌肉粗脂质和糖原含量呈显著的二阶多项式关系 (P < 0.05)。全身和肝脏的肝体指数、肠系膜脂肪指数、肝糖原含量和粗脂质含量呈显著线性和二阶多项式关系 (P < 0.05)。基于 WGR、SGR、PER 和 FCR 的二次曲线模型分析表明,在尼罗罗非鱼的 HCD 中,琥珀酸的最佳补充范围为 1.83% 至 2.43%。与对照组相比,饲喂 1.0% 琥珀酸的鱼具有较高的肌肉硬度,胶原蛋白中碱溶性羟脯氨酸、二十二碳六烯酸 (DHA) 和 n-3 多不饱和脂肪酸 (n-3PUFA) 含量增加,肌肉中总脂肪酸含量降低 (P < 0.05)。与对照组相比,膳食补充剂 1.0 %琥珀酸显著提高肌肉中总结合氨基酸(精氨酸、组氨酸、异亮氨酸、赖氨酸、蛋氨酸、丙氨酸、脯氨酸)、总风味氨基酸(游离天冬氨酸)、过氧化氢酶(CAT)活性和总抗氧化能力,以及肌中CAT、超氧化物歧化酶(SOD)和核因子红细胞衍生的2样2(Nrf2)的mRNA相对表达水平(P < 0.05)。此外,补充琥珀酸显著上调了糖酵解基因(己糖激酶 2 [HK2]、磷酸果糖激酶、肌-A [PFKMA] 和磷酸果糖激酶、肌 B [PFKMB])、关键糖原合成基因(糖原合酶 [GYS])和脂质分解代谢基因(肉碱棕榈酰转移酶-1B [CPT1B]、激素敏感脂肪酶 [HSL] 和脂蛋白脂肪酶 [LPL])的 mRNA 相对表达水平,同时下调脂肪酸合酶的 mRNA 相对表达水平(FASN) 在肌肉中 (P < 0.05)。总之,膳食中添加 1.83% 至 2.43% 琥珀酸通过增加肌肉抗氧化能力和硬度、改变肌肉氨基酸和脂肪酸组成以及调节肌肉葡萄糖和脂质代谢来改善肌肉质量。
更新日期:2024-05-25
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