Methyleugenol is widely found in essential oils from aromatic plants, attracting insects for pollination and possessing antimicrobial and antioxidant features. Methyleugenol content in the essential oil from leaves is 90.46 %, making it an excellent material for assessing methyleugenol’s biosynthetic pathway. Eugenol synthase (EGS) and eugenol-o-methyltransferase (EOMT) represent key genes in methyleugenol’s biosynthetic pathway but their respective functions are unclear. In this study, overexpression and virus-induced gene silencing (VIGS) techniques were applied to investigate the major regulatory gene among , , , and in the biosynthesis pathway of methyleugenol. We also aimed to examine the coordination mechanism between and in methyleugenol production by . The data revealed transcription and methyleugenol content were most significantly increased after overexpression of the gene in and . Conversely, transcription and methyleugenol content were most significantly reduced after gene silencing by VIGS in . These results underscore a major regulatory role for the gene in the biosynthetic pathway of methyleugenol in . Remarkably, upon overexpression and silencing of , the transcription levels of were notably upregulated, with showing the highest increase. Collectively, these findings suggested the presence of a specific transcription factor bound to the promoters of and , inducing their expression for the synergistic and efficient production of methyleugenol in .

中文翻译：

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MbEGS4与MbEOMT在白千层F. Muell合成甲基丁香酚中的协调机制分析


甲基丁香酚广泛存在于芳香植物精油中，可吸引昆虫授粉，并具有抗菌和抗氧化功能。叶子精油中甲基丁香酚含量为90.46%，使其成为评估甲基丁香酚生物合成途径的极好材料。丁子香酚合成酶（EGS）和丁香酚邻位甲基转移酶（EOMT）是甲基丁香酚生物合成途径中的关键基因，但它们各自的功能尚不清楚。本研究采用过表达和病毒诱导基因沉默（VIGS）技术来研究甲基丁香酚生物合成途径中的主要调控基因 、 、 、 和 。我们还旨在研究甲基丁香酚生产之间的协调机制。数据显示，在 和 中过度表达该基因后，转录和甲基丁香酚含量最显着增加。相反，在 中，通过 VIGS 基因沉默后，转录和甲基丁香酚含量显着降低。这些结果强调了该基因在甲基丁香酚生物合成途径中的主要调节作用。值得注意的是，在 的过表达和沉默后， 的转录水平显着上调，且增幅最高。总的来说，这些发现表明存在与 和 的启动子结合的特定转录因子，诱导它们的表达，从而在 中协同和有效地生产甲基丁香酚。