Root-knot nematodes (RKNs) pose a significant threat to global crop production. Given the detrimental effects of fumigants and conventional chemical nematicides, there is a growing interest in exploring biological nematicides as a promising alternative for pest control. In this study, we present data demonstrating the role of root volatiles in enhancing resistance against () in tobacco plants overexpressing (-OE). The gene, derived from , encodes a 1,8-cineole synthase induced by infection and predominantly localized to the chloroplast. The roots of -OE plants exhibited deterrent properties to infective second-stage juveniles (J2s) of . Moreover, -OE plants showed enhanced resistance against , as evidenced by reduced gall numbers and egg mass counts compared to wild type (WT) plants. Notably, the emission of root volatiles from -OE plants, including 1,8-cineole, α-terpineol, terpinene-4-ol, and furfural, was significantly higher than that of WT. assays confirmed that a mixture of commercially available volatile organic compounds (VOCs) had a deterrent effect on infective J2s. Furthermore, the natural root volatiles emitted by -OE plants effectively enhanced tobacco's resistance against . Our findings provide valuable insights into the role of in volatile-based defense and highlight the potential of metabolic engineering of monoterpene synthase genes to control by enhancing root volatile emissions.

中文翻译：

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来自烟草的 1,8-桉树脑合酶的代谢工程，通过根挥发物的协同混合增强对南方根结线虫的抗性


根结线虫（RKN）对全球农作物生产构成重大威胁。鉴于熏蒸剂和传统化学杀线虫剂的有害影响，人们越来越有兴趣探索生物杀线虫剂作为害虫控制的有前途的替代品。在这项研究中，我们提供的数据证明了根挥发物在增强过度表达（-OE）的烟草植物中对（）的抗性中的作用。该基因源自 ，编码由感染诱导的 1,8-桉树脑合酶，主要定位于叶绿体。 -OE 植物的根对 .此外，与野生型 (WT) 植物相比，-OE 植物表现出增强的抗性，其虫瘿数量和卵质量计数减少就证明了这一点。值得注意的是，-OE植物根系挥发物的排放量，包括1,8-桉叶素、α-萜品醇、萜品烯-4-醇和糠醛，显着高于WT。分析证实，市售挥发性有机化合物 (VOC) 的混合物对感染性 J2 具有威慑作用。此外，-OE植物释放的天然根部挥发物有效增强了烟草的抗性。我们的研究结果为了解挥发性防御中的作用提供了宝贵的见解，并强调了单萜合酶基因代谢工程通过增强根挥发性排放来控制的潜力。