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The Wild West of spike-in normalization
Nature Biotechnology ( IF 33.1 ) Pub Date : 2024-09-13 , DOI: 10.1038/s41587-024-02377-y
Lauren A Patel 1, 2, 3 , Yuwei Cao 3, 4 , Eric M Mendenhall 5 , Christopher Benner 2 , Alon Goren 3
Affiliation  

The proper use of spike-in normalization in ChIP-seq improves sensitivity for detecting genome-wide changes between conditions, but improper use is common, calling some biological conclusions into question. A survey of public datasets generates guidelines for implementation of spike-in normalization for future ChIP-seq experiments.

中文翻译:


标准化的狂野西部



在 ChIP-seq 中正确使用 spike-in 标准化可提高检测条件之间全基因组变化的灵敏度,但使用不当的情况很常见,使一些生物学结论受到质疑。对公共数据集的调查为未来 ChIP-seq 实验的尖峰标准化实施提供了指南。
更新日期:2024-09-13
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