Aflatoxin B1 (AFB1) is a potent carcinogen, and is among the most hazardous mycotoxins in agricultural products. Therefore, the development of sensitive and convenient detection methods for AFB1 is significant for food safety against mycotoxins. Herein, a bioluminescent enzyme immunoassay (BLEIA) was developed for ultrasensitive detection of AFB1, based on the novel Fc-specific antibody–nanoluciferase (Ab–Nluc) conjugates which were fabricated using an IgG-binding protein-assisted photo-conjugation strategy. In indirect competitive immunoassay format, the proposed BLEIA exhibited the detection limit of 0.0232 ng mL−1, which was 37.4-fold lower than that obtained using the classical enzyme-linked immunosorbent assay (ELISA) based on Ab–horseradish peroxidase (Ab–HRP) chemical conjugates (0.868 ng mL−1). Meanwhile, the BLEIA exhibited high accuracy and precision. Thus, the proposed Fc-specific Ab–Nluc conjugates-based BLEIA provides an ultrasensitive and reliable method for detecting toxins and has potential for use in food safety monitoring.

中文翻译：

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基于 Fc 特异性偶联抗体-纳米荧光素酶免疫试剂的生物发光免疫测定法的开发，用于测定黄曲霉毒素 B1


黄曲霉毒素 B1 （AFB1） 是一种强致癌物，是农产品中最危险的霉菌毒素之一。因此，开发灵敏、便捷的 AFB1 检测方法对霉菌毒素的食品安全具有重要意义。在此，开发了一种生物发光酶免疫测定法 （BLEIA），用于 AFB1 的超灵敏检测，基于使用 IgG 结合蛋白辅助光偶联策略制造的新型 Fc 特异性抗体-纳米荧光素酶 （Ab-Nluc） 偶联物。在间接竞争免疫测定形式中，拟议的 BLEIA 的检测限为 0.0232 ng mL-1，比使用基于 Ab-辣根过氧化物酶 （Ab-HRP） 化学偶联物的经典酶联免疫吸附测定 （ELISA） 获得的检测限低 37.4 倍（0.868 ng mL-1）。同时，BLEIA 表现出高准确度和精密度。因此，拟议的基于 Fc 特异性 Ab-Nluc 偶联物的 BLEIA 提供了一种超灵敏和可靠的毒素检测方法，并具有用于食品安全监测的潜力。