The extraction methods for antimicrobial peptides (AMPs) from plants are varied, but the absence of a standardized and rapid technique remains a challenge. In this study, a functionalized biochar was developed and characterized for the extraction of AMPs from pea protein hydrolysates. The results indicated that the biochar mainly enriched AMPs through electrostatic interaction, hydrogen bonding and pore filling. Then three novel cationic antimicrobial peptides were identified, among which the RDLFK (Arg-Asp-Leu-Phe-Lys) had the greatest inhibitory effect against Staphylococcus aureus and Bacillus subtilis, showcasing IC50 value of 2.372 and 1.000 mg/mL, respectively. Additionally, it was found that RDLFK could damage bacterial cell membranes and penetrate the cells to inhibit DNA synthesis. These results provided that the biochar-based extraction method presents an efficient and promising avenue for isolating AMPs, addressing a critical gap in the current methodologies for their extraction from plant sources.

中文翻译：

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磺酸功能化生物炭从豌豆蛋白水解物中提取抗菌肽


从植物中提取抗菌肽 （AMP） 的方法多种多样，但缺乏标准化和快速的技术仍然是一个挑战。在这项研究中，开发了一种功能化生物炭并进行了表征，用于从豌豆蛋白水解物中提取 AMPs。结果表明，生物炭主要通过静电相互作用、氢键和孔隙填充来富集 AMPs。然后鉴定出 3 种新型阳离子抗菌肽，其中 RDLFK（Arg-Asp-Leu-Phe-Lys）对金黄色葡萄球菌和枯草芽孢杆菌的抑制作用最大，IC50 值分别为 2.372 和 1.000 mg/mL。此外，研究发现 RDLFK 可破坏细菌细胞膜并渗透细胞以抑制 DNA 合成。这些结果表明，基于生物炭的提取方法为分离 AMP 提供了一种有效且有前途的途径，解决了当前从植物来源提取 AMP 的方法中的关键差距。