Nanozyme-Based Microfluidic Chip System for pH-Regulated Pretreatment and Sensitive Sensing,Analytical Chemistry

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Nanozyme-Based Microfluidic Chip System for pH-Regulated Pretreatment and Sensitive Sensing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-13 , DOI: 10.1021/acs.analchem.4c02415
Yifei Chen ₁ , Yu Wu ₁ , Weiqing Xu ₁ , Yinjun Tang ₁ , Yujia Cai ₁ , Xin Yu ₁ , Jian Li ₁ , Yiwei Qiu ₁ , Liuyong Hu ₂ , Wenling Gu ₁ , Chengzhou Zhu _{1,

3}

Affiliation

Nanozymes, possessing nanomaterial properties and catalytic activities, offer great opportunities to design sensitive analytical detection systems. However, the low interference resistance of nanozymes poses a significant limitation on the precise detection of target substances. Herein, a nanozyme-based microfluidic chip system for pH-regulated pretreatment and sensitive sensing of cysteine (Cys) is reported. The copper metal–organic framework (Cu MOF) exhibits good cysteine oxidase-like activity at pH 7.0, while demonstrating excellent laccase-like activity at pH 8.0. Taking advantage of the pH-regulated enzyme-like activity, the integrated microfluidic device involving the immobilization of Cu MOF eliminates the interference of dopamine (DA) and accurately detects the target Cys. Compared with the untreated reaction system, the developed nanozyme system shows a significantly improved accuracy in detecting Cys, with an R² value of 0.9914. This work provides an efficient method to enhance the interference resistance of nanozymes and broadens the application in sample pretreatment.

中文翻译：

基于纳米酶的微流控芯片系统，用于 pH 调节预处理和灵敏传感

纳米酶具有纳米材料特性和催化活性，为设计灵敏的分析检测系统提供了绝佳的机会。然而，纳米酶的低干扰性对目标物质的精确检测造成了很大的限制。本文报道了一种基于纳米酶的微流控芯片系统，用于 pH 调节预处理和半胱氨酸 (Cys) 的灵敏传感。铜金属有机框架（Cu MOF）在 pH 7.0 下表现出良好的半胱氨酸氧化酶样活性，同时在 pH 8.0 下表现出优异的漆酶样活性。利用pH调节的类酶活性，固定Cu MOF的集成微流控装置消除了多巴胺（DA）的干扰，准确检测目标Cys。与未处理的反应体系相比，所开发的纳米酶体系检测Cys的准确度显着提高， R ²值为0.9914。该工作为增强纳米酶的抗干扰性提供了一种有效的方法，拓宽了纳米酶在样品前处理中的应用。

更新日期：2024-09-13

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