Electro-responsive Molecularly Imprinted Polymers (E-MIPs) for Rapid Detection of Progesterone in Human Serum Samples,Analytical Chemistry

当前位置： X-MOL 学术 › Anal. Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Electro-responsive Molecularly Imprinted Polymers (E-MIPs) for Rapid Detection of Progesterone in Human Serum Samples
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-13 , DOI: 10.1021/acs.analchem.4c02417
Siva Ananth Mariappan _{1,

2} , Pandiaraj Manickam _{1,

2}

Affiliation

Rapid and reagent-free detection of progesterone (P4) is crucial in point-of-care (POC) measurement due to its important role in the human endocrine and central nervous system. Currently available technologies for P4 detection are often not rapid or require reagents, limiting their use in POC settings. In this work, a self-signaling electrochemical sensing platform for rapid detection of P4 is developed by using electroactive molecularly imprinted polymers (E-MIPs). E-MIPs possess the ability to transduce the molecular recognition event into a measurable electrochemical signal. The E-MIPs are prepared by bulk copolymerization of acrylic acid (AA) and ferrocenylmethyl methacrylate (FcMA) in the presence of P4 followed by its removal from the polymer matrix. By incorporation of ferrocene moieties, the MIP gains intrinsic electroactivity, enabling label-free and direct electrochemical detection of P4 levels. Electrochemical techniques, including cyclic voltammetry and electrochemical impedance spectroscopy, were employed to elucidate the electron transport reaction responsible for the MIP’s intrinsic electroactivity. Additionally, the predetermined complementary binding sites confined to the small working area of the screen-printed carbon electrode (SPCE) within the E-MIP matrix facilitate rapid P4 binding, achieving completion within 120 s. The developed E-MIP sensing platform was used for the rapid detection of P4 levels in human serum samples. The short incubation times reported for the E-MIP sensing platform would be beneficial in minimizing the nonspecific binding in the real-world samples. We believe that the proposed E-MIP sensing platform can be applied to clinical samples for detecting fluctuations in P4 levels.

中文翻译：

用于快速检测人血清样品中孕酮的电响应分子印迹聚合物 (E-MIP)

由于孕酮 (P4) 在人类内分泌和中枢神经系统中发挥着重要作用，因此快速、无试剂检测孕酮 (P4) 在即时护理 (POC) 测量中至关重要。目前可用的 P4 检测技术通常速度不快或需要试剂，限制了它们在 POC 环境中的使用。在这项工作中，利用电活性分子印迹聚合物（E-MIP）开发了一种用于快速检测 P4 的自信号电化学传感平台。 E-MIP 具有将分子识别事件转换为可测量的电化学信号的能力。 E-MIP 是通过丙烯酸 (AA) 和甲基丙烯酸二茂铁甲酯 (FcMA) 在 P4 存在下进行本体共聚，然后将其从聚合物基质中去除而制备的。通过掺入二茂铁部分，MIP 获得了固有的电活性，从而实现了 P4 水平的无标记和直接电化学检测。采用循环伏安法和电化学阻抗谱等电化学技术来阐明导致 MIP 固有电活性的电子传输反应。此外，预定的互补结合位点局限于 E-MIP 矩阵内丝网印刷碳电极 (SPCE) 的小工作区域，有利于 P4 的快速结合，在 120 秒内完成。开发的E-MIP传感平台用于快速检测人血清样本中的P4水平。 E-MIP 传感平台的短孵育时间将有利于最大限度地减少现实样品中的非特异性结合。我们相信所提出的 E-MIP 传感平台可以应用于临床样本以检测 P4 水平的波动。

更新日期：2024-09-13

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南