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Drip-Dry Strategy Assisted Blu-Ray Disc Biosensor for Fast Point of Care Testing
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-13 , DOI: 10.1021/acs.analchem.4c02651 Yunhao Zhang 1 , Wenjie Lv 1 , Zixin Kang 1 , Anxin Guo 1 , Junming Li 1 , Chenxi Dai 1 , Mingyu Zhang 1 , Siyu Gao 1 , Shunji Li 1 , Zeyu Miao 1 , Sihan Chen 1 , Xiaojun Feng 1 , Yiwei Li 1 , Peng Chen 1 , Bi-Feng Liu 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-13 , DOI: 10.1021/acs.analchem.4c02651 Yunhao Zhang 1 , Wenjie Lv 1 , Zixin Kang 1 , Anxin Guo 1 , Junming Li 1 , Chenxi Dai 1 , Mingyu Zhang 1 , Siyu Gao 1 , Shunji Li 1 , Zeyu Miao 1 , Sihan Chen 1 , Xiaojun Feng 1 , Yiwei Li 1 , Peng Chen 1 , Bi-Feng Liu 1
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Discs and numerous other consumer products have been developed for point of care testing (POCT) to replace traditional large and expensive biochemical devices in certain scenarios. Herein, we propose a drip-dry strategy (2D strategy) assisted Blu-ray disc (BD) biosensor, termed BDB, for rapid and portable POCT within 30 min with the cost of a single test < $1. The platform utilizes the covered area formed by the deposition of the substance to be measured on the activated BD surface after the evaporation of water and realizes the quantitative detection of the target through the error readout of free disc quality diagnosis software. As a proof of concept, we first demonstrated the feasibility of direct quantitative detection of substances in solution in a single system through the detection of pure proteins avoiding colorimetric reagent used in traditional optical detection. For the complex mixed systems, we then innovatively utilize the principle that soluble targets promote/inhibit the dissolution of insoluble precipitates to achieve specific detection of targets and successfully apply BDB to the indirect quantitative detection of glutathione (GSH) with LOD of 0.447 mM in the range of 2–16 mM and organophosphorus pesticides (OPs) with LOD of 2.122 × 10–7 M in the range of 1.289 × 10–7–1.289 × 10–4 M. The BDB is widely applicable, easy to operate, and less time-consuming, which is anticipated to provide an alternative method for early, on-site detection or screening.
中文翻译：
滴干策略辅助蓝光光盘生物传感器，用于快速护理测试
光盘和许多其他消费产品已开发用于即时检测 (POCT)，以在某些情况下取代传统的大型且昂贵的生化设备。在此，我们提出了一种滴干策略（2D 策略）辅助蓝光光盘（BD）生物传感器，称为 BDB，用于 30 分钟内快速便携式 POCT，单次测试的成本为 < $1。该平台利用待测物质在水分蒸发后沉积在活化的BD表面上形成的覆盖区域，通过免费光盘质量诊断软件的误差读数，实现对目标物的定量检测。作为概念证明，我们首先证明了通过检测纯蛋白质来直接定量检测单个系统中溶液中物质的可行性，避免了传统光学检测中使用的比色试剂。针对复杂的混合体系，我们创新性地利用可溶性靶标促进/抑制不溶性沉淀物溶解的原理，实现靶标的特异性检测，并成功将BDB应用于谷胱甘肽（GSH）的间接定量检测，检测限为0.447 mM。 BDB 的检测范围为 2–16 mM，LOD 为 2.122 × 10 –7 M 的有机磷农药 (OPs) 的范围为 1.289 × 10 –7 –1.289 × 10 –4 M。 BDB 适用范围广、操作方便、成本低。耗时，预计这将为早期现场检测或筛查提供替代方法。
更新日期：2024-09-13
中文翻译：
滴干策略辅助蓝光光盘生物传感器，用于快速护理测试
光盘和许多其他消费产品已开发用于即时检测 (POCT)，以在某些情况下取代传统的大型且昂贵的生化设备。在此，我们提出了一种滴干策略（2D 策略）辅助蓝光光盘（BD）生物传感器，称为 BDB，用于 30 分钟内快速便携式 POCT，单次测试的成本为 < $1。该平台利用待测物质在水分蒸发后沉积在活化的BD表面上形成的覆盖区域，通过免费光盘质量诊断软件的误差读数，实现对目标物的定量检测。作为概念证明，我们首先证明了通过检测纯蛋白质来直接定量检测单个系统中溶液中物质的可行性，避免了传统光学检测中使用的比色试剂。针对复杂的混合体系，我们创新性地利用可溶性靶标促进/抑制不溶性沉淀物溶解的原理，实现靶标的特异性检测，并成功将BDB应用于谷胱甘肽（GSH）的间接定量检测，检测限为0.447 mM。 BDB 的检测范围为 2–16 mM，LOD 为 2.122 × 10 –7 M 的有机磷农药 (OPs) 的范围为 1.289 × 10 –7 –1.289 × 10 –4 M。 BDB 适用范围广、操作方便、成本低。耗时，预计这将为早期现场检测或筛查提供替代方法。
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