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Glucose Oxidase-Based Glucose Biosensing with a Simple Dual Ag/AgCl Probe Conductivity Readout
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-12 , DOI: 10.1021/acs.analchem.4c03088 Kawin Loha 1 , H Sasimali M Soysa 1, 2 , Thitikorn Boonkoom 3 , Deanpen Japrung 3 , Albert Schulte 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-12 , DOI: 10.1021/acs.analchem.4c03088 Kawin Loha 1 , H Sasimali M Soysa 1, 2 , Thitikorn Boonkoom 3 , Deanpen Japrung 3 , Albert Schulte 1
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We describe a conductometric assay of the enzymatic conversion of glucose to gluconic acid by dissolved glucose oxidase (GOx), using the generation of proton and gluconate from the reaction product dissociation for glucose detection. Simple basics of ionic conductivity, a silver/silver chloride wire pair, and a small applied potential translate glucose-dependent GOx activity into a scalable cell current. Enzyme immobilization and complex sensor design, involving extra nanomaterials or microfabrication of electrode structures, are entirely avoided, in contrast to all modern electrochemical glucose biosensors. Assay calibration showed a response linearity up to 500 μM, with a sensitivity of about 1.3 nA/μM. Selectivity tests excluded signals from sugars other than glucose, and glucose quantifications with recovery rates close to 100% were reached with a model sample and a beverage. Easy use of elementary physicochemical phenomena and a satisfactory performance are assets of the proposed non-amperometric glucose biosensing strategy. Assay integration into a planar dual electrode platform, with or without microfluidic application option, is feasible because of the simplicity of the sensor readout and suggests a route to affordable glucose analysis in beverage, food, and body fluid samples.
中文翻译：
基于葡萄糖氧化酶的葡萄糖生物传感，具有简单的双 Ag/AgCl 探针电导率读数
我们描述了通过溶解的葡萄糖氧化酶（GOx）将葡萄糖酶促转化为葡萄糖酸的电导测定，利用反应产物解离产生质子和葡萄糖酸盐进行葡萄糖检测。离子电导率的简单基础知识、银/氯化银线对和小的外加电位将葡萄糖依赖性 GOx 活性转化为可扩展的细胞电流。与所有现代电化学葡萄糖生物传感器相比，完全避免了酶固定和复杂的传感器设计，包括额外的纳米材料或电极结构的微加工。测定校准显示响应线性高达 500 μM，灵敏度约为 1.3 nA/μM。选择性测试排除了除葡萄糖以外的糖类信号，模型样品和饮料的葡萄糖定量回收率接近 100%。基本物理化学现象的简单使用和令人满意的性能是所提出的非安培葡萄糖生物传感策略的优点。由于传感器读数简单，将分析集成到平面双电极平台（无论有或没有微流体应用选项）都是可行的，并提出了一种在饮料、食品和体液样品中进行经济实惠的葡萄糖分析的途径。
更新日期：2024-09-12
中文翻译：
基于葡萄糖氧化酶的葡萄糖生物传感，具有简单的双 Ag/AgCl 探针电导率读数
我们描述了通过溶解的葡萄糖氧化酶（GOx）将葡萄糖酶促转化为葡萄糖酸的电导测定，利用反应产物解离产生质子和葡萄糖酸盐进行葡萄糖检测。离子电导率的简单基础知识、银/氯化银线对和小的外加电位将葡萄糖依赖性 GOx 活性转化为可扩展的细胞电流。与所有现代电化学葡萄糖生物传感器相比，完全避免了酶固定和复杂的传感器设计，包括额外的纳米材料或电极结构的微加工。测定校准显示响应线性高达 500 μM，灵敏度约为 1.3 nA/μM。选择性测试排除了除葡萄糖以外的糖类信号，模型样品和饮料的葡萄糖定量回收率接近 100%。基本物理化学现象的简单使用和令人满意的性能是所提出的非安培葡萄糖生物传感策略的优点。由于传感器读数简单，将分析集成到平面双电极平台（无论有或没有微流体应用选项）都是可行的，并提出了一种在饮料、食品和体液样品中进行经济实惠的葡萄糖分析的途径。
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