Synergistic Effect of Two Peptaibols from Biocontrol Fungus Trichoderma longibrachiatum Strain 40418 on CO-Induced Plant Resistance,Journal of Agricultural and Food Chemistry

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Synergistic Effect of Two Peptaibols from Biocontrol Fungus Trichoderma longibrachiatum Strain 40418 on CO-Induced Plant Resistance
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-13 , DOI: 10.1021/acs.jafc.4c01952
Ning Luo _{1,

2} , Yang Jiao _{1,

3} , Jian Ling ₁ , Zeyu Li ₁ , Wenwen Zhang ₁ , Jianlong Zhao ₁ , Yan Li ₁ , Zhenchuan Mao ₁ , Huixia Li ₂ , Bingyan Xie _{1,

4}

Affiliation

Trichoderma longibrachiatum is a filamentous fungus used as a biological control agent against different plant diseases. The multifunctional secondary metabolites synthesized by Trichoderma, called peptaibols, have emerged as key elicitors in plant innate immunity. This study obtained a high-quality genome sequence for the T. longibrachiatum strain 40418 and identified two peptaibol biosynthetic gene clusters using knockout techniques. The two gene cluster products were confirmed as trilongin AIV a (11-residue) and trilongin BI (20-residue) using liquid chromatography coupled with tandem mass spectrometry. Further investigations revealed that these peptaibols induce plant resistance to Pseudomonas syringae pv tomato (Pst) DC3000 infection while triggering plant immunity and cell death. Notably, the two peptaibols exhibit synergistic effects in plant-microbe signaling interactions, with trilongin BI having a predominant role. Moreover, the induction of tomato resistance against Meloidogyne incognita showed similarly promising results.

中文翻译：

生防真菌长枝木霉菌株 40418 的两种 Peptaibols 对 CO 诱导的植物抗性的协同作用

长枝木霉(Trichoderma longibrachiatum)是一种丝状真菌，用作针对不同植物病害的生物防治剂。木霉合成的多功能次级代谢产物（称为肽醇）已成为植物先天免疫的关键诱导子。本研究获得了T. longibrachiatum菌株 40418 的高质量基因组序列，并利用敲除技术鉴定了两个 peptaibol 生物合成基因簇。使用液相色谱-串联质谱法确认这两个基因簇产物为trilongin AIV a（11个残基）和trilongin BI（20个残基）。进一步的研究表明，这些 peptaibols 诱导植物对番茄致病变种丁香假单胞菌( Pst ) DC3000 感染产生抗性，同时触发植物免疫和细胞死亡。值得注意的是，这两种 peptaibol 在植物-微生物信号相互作用中表现出协同效应，其中 trilongin BI 起主导作用。此外，诱导番茄对南方根结线虫的抗性也显示出类似的有希望的结果。

更新日期：2024-09-13

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