ObjectivesThis study aimed to explore multiple effects of hyperbranched poly‐l‐lysine (HBPL) titanium (Ti) surfaces on osseointegration, bacteriostasis, and anti‐inflammation across three different animal models.MethodsTi surfaces were covalently modified with HBPL, with uncoated surfaces as controls. Characterization included scanning electron microscopy (SEM) and surface chemistry and elemental analysis (EDX). Ti and Ti‐HBPL implants were placed in conventional canine edentulous sites, post‐operative infection canine edentulous sites, and diabetic rat tibias. Implants from canine edentulous models were analyzed using micro‐CT and histomorphometry to assess osseointegration at 8 weeks. Post‐operative infection beagles were used to evaluate antibacterial efficacy through clinical parameters and bacterial cultures at 1 week. In diabetic rats, micro‐CT and histomorphometry were performed at 8 weeks.ResultsHBPL was uniformly grafted on Ti‐HBPL surfaces. Ti‐HBPL surfaces showed higher bone volume/total volume (BV/TV, p < 0.001), bone‐implant contact (BIC%, p < 0.001), and trabecular number (Tb.N, p < 0.01) in beagles. Besides, it displayed higher BIC% (p < 0.001) and bone area fraction occupancy (BAFO%, p < 0.01) in hard tissue sections. In an infected model, Ti‐HBPL surfaces exhibited lower bleeding on probing (BOP, p < 0.001), and plaque index (DI, p < 0.01), with reduced bacterial colony formation (p < 0.001) compared to the control group. In diabetic rats, Ti‐HBPL surfaces showed an increase in BV/TV (p < 0.01) and Tb.N (p < 0.001), downregulated TNF‐α and IL‐1β (p < 0.01), and upregulated IL‐10 (p < 0.01) and osteocalcin (OCN) expression (p < 0.01).ConclusionsHBPL‐Ti surfaces demonstrated enhanced osseointegration, bacteriostasis, and anti‐inflammatory effects in vivo.

中文翻译：

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具有增强骨整合、抑菌和抗炎特性的超支化聚赖氨酸修饰钛表面，用于植入物应用：体内实验研究


目的本研究旨在探讨超支化聚赖氨酸（HBPL）钛（Ti）表面对三种不同动物模型的骨整合、抑菌和抗炎的多重影响。方法用HBPL对Ti表面进行共价修饰，以未涂层表面作为对照。表征包括扫描电子显微镜 (SEM) 以及表面化学和元素分析 (EDX)。 Ti 和 Ti-HBPL 种植体被放置在常规犬无牙颌部位、术后感染犬无牙颌部位和糖尿病大鼠胫骨中。使用显微 CT 和组织形态计量学对犬无牙颌模型的种植体进行分析，以评估第 8 周时的骨整合情况。使用术后感染比格犬，通过临床参数和 1 周细菌培养来评估抗菌效果。在糖尿病大鼠中，8周时进行显微CT和组织形态测定。结果HBPL均匀地移植到Ti-HBPL表面。 Ti-HBPL 表面显示出更高的骨体积/总体积 (BV/TV, p < 0.001)、骨种植体接触 (BIC%, p < 0.001) 和小梁数量 (Tb.N, p < 0.01)比格犬。此外，它在硬组织切片中显示出较高的 BIC% (p < 0.001) 和骨面积分数占有率 (BAFO%, p < 0.01)。在感染模型中，与对照相比，Ti-HBPL 表面在探测时表现出较低的出血量 (BOP, p < 0.001) 和菌斑指数 (DI, p < 0.01)，并且细菌菌落形成减少 (p < 0.001)团体。在糖尿病大鼠中，Ti-HBPL 表面显示 BV/TV (p < 0.01) 和 Tb.N (p < 0.001) 增加，TNF-α 和 IL-1β 下调 (p < 0.01)，IL 上调‐10 (p < 0.01) 和骨钙素 (OCN) 表达 (p < 0.01)。结论HBPL-Ti 表面在体内具有增强的骨整合、抑菌和抗炎作用。