Environmental stressors induce specific physiological responses that can be measured in the blood, notably by morphological changes in lymphocytes. Tobacco being the best-known stress in terms of its impact on health, we studied the physiological properties of peripheral blood lymphocytes in a population of 33 healthy non-smokers and smokers. Proteasome amount, mitochondria energy levels, changes in membrane properties and cell and nuclear size were analyzed to obtain 28 parameters from two fluorescence-based techniques: flow cytometry and cell imaging. The results showed that none of the parameters alone identified gender and smoking status, but that statistical analysis of these parameters, whether or not combined with a third set of data, hematological data, can. Statistical analysis of selected parameters clearly discriminates between male and female samples, as well as smokers and non-smokers. Effects of tobacco smoke pollutants are more pronounced in female smokers than in other groups.

中文翻译：

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综合多尺度分析揭示了吸烟者淋巴细胞中复杂的性别特异性变化


环境压力源会诱导特定的生理反应，这些反应可以在血液中测量，特别是通过淋巴细胞的形态变化。就烟草对健康的影响而言，烟草是最著名的压力，我们研究了 33 名健康非吸烟者和吸烟者人群的外周血淋巴细胞的生理特性。分析蛋白酶体数量、线粒体能量水平、膜特性以及细胞和细胞核大小的变化，从两种基于荧光的技术中获得 28 个参数：流式细胞术和细胞成像。结果表明，没有一个参数单独确定性别和吸烟状况，但对这些参数的统计分析，无论是否与第三组数据（血液学数据）相结合，都可以。对所选参数的统计分析清楚地区分了男性和女性样本，以及吸烟者和非吸烟者。烟草烟雾污染物的影响在女性吸烟者中比其他群体更明显。