Soup is an important presence in diet, but its absorption and transport mechanism by the human body remains unclear. In this study, Caco-2 intact cell and monolayer cell models were constructed to simulate small intestine absorption on colloidal nanoparticles (CNPs) isolated from lamb soup. The intracellular localization of CNPs was viewed by laser confocal microscopy (LSCM). CNPs uptake and release pathways were explored by differences in CNPs concentrations in 5 endocytosis inhibitor models and 4 exocytosis inhibitor models. Results indicated that CNPs endocytosis by Caco-2 cells was restrained by Nystatin and Cytochalasin D, with exocytosis being inhibited by Nocodazole and Monensin. Therefore, the major absorption pathways for CNPs were caveolin-dependent endocytosis, macropinocytosis and phagocytosis. The major transport pathways were microtubule-vesicle-mediated protein transport to the membrane and transportation between the Golgi apparatus and membrane. This study may provide theoretical support for the transport mechanism of soup products in the small intestine.

中文翻译：

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基于 Caco-2 细胞的胶体纳米颗粒 （CNP） 在羊汤中的吸收和转运机制


汤是饮食中的重要存在，但其被人体吸收和运输的机制仍不清楚。在这项研究中，构建了 Caco-2 完整细胞和单层细胞模型，以模拟小肠对从羊汤中分离的胶体纳米颗粒 （CNP） 的吸收。通过激光共聚焦显微镜 （LSCM） 观察 CNPs 的细胞内定位。通过 5 种内吞作用抑制剂模型和 4 种胞吐作用抑制剂模型中 CNPs 浓度的差异来探索 CNPs 的摄取和释放途径。结果表明，制霉菌素和细胞松弛素 D 抑制了 Caco-2 细胞的 CNPs 内吞作用，诺考达唑和莫能菌素抑制了胞吐作用。因此，CNPs 的主要吸收途径是小窝蛋白依赖性内吞作用、巨胞饮作用和吞噬作用。主要的运输途径是微管-囊泡介导的蛋白质向膜的运输以及高尔基体与膜之间的运输。本研究可为汤品在小肠内的转运机制提供理论支持。