The threat posed by organophosphorus pesticides (OPS) to food safety, human health, and the ecological environment is significant, which underscoring the need for the development of new detection tools. We designed and synthesized a NIR fluorescent probe PT-CES which targets carboxylesterase (CES), for the detection of OPS based on the principle of enzyme inhibition. The PT-CES is capable of instantaneous response to CES, exhibiting excellent stability, anti-interference capability. PT-CES realizes the quantitative detection of CES and OPS. It is noteworthy that PT-CES shows excellent stable and accurate detection ability in vegetable pesticide testing. It also enables the monitoring of CES activity in cells and liver tissue. This provides a novel tool for tracking the effect of OPS on CES activity in biological systems. Furthermore, it provides a useful method for ensuring food safety and enhancing pesticide residue analysis.

中文翻译：

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一种新型超快、高灵敏度的 NIR 荧光探针，用于检测食品和生物系统中的有机磷农药


有机磷农药 （OPS） 对食品安全、人类健康和生态环境构成的威胁是巨大的，这凸显了开发新检测工具的必要性。我们设计合成了一种靶向羧酸酯酶 （CES） 的 NIR 荧光探针 PT-CES，用于基于酶抑制原理检测 OPS。PT-CES 能够对 CES 进行瞬时响应，表现出优异的稳定性、抗干扰能力。PT-CES 实现了 CES 和 OPS 的定量检测。值得注意的是，PT-CES 在蔬菜农药检测中表现出优异的稳定和准确的检测能力。它还能够监测细胞和肝组织中的 CES 活性。这为跟踪 OPS 对生物系统中 CES 活性的影响提供了一种新工具。此外，它还为确保食品安全和加强农药残留分析提供了一种有用的方法。