Autotoxicity stress is the principal factor in peach replant problem. Benzoic acid (BA) is known as a critical autotoxin in replant problem, and causes an obvious inhibitory effect on peach growth. Small heat shock proteins (sHSPs) have been reported to play pivotal roles in a variety of physiological and biological processes in various plants. Nevertheless, little is known about the functions and the underlying physiological mechanisms of under autotoxicity stress. Here, we identified of peach () and deciphered its role in BA stress response. was significantly induced by BA treatment. Overexpression of elevated BA tolerance in and peach plants, whereas down-regulation of in peach through virus-induced gene silencing enhanced BA sensitivity. Compared to the control, the overexpressing plants exhibited lower contents of reactive oxygen species (ROS) and higher activities of antioxidant enzymes. Furthermore, regulated the transcripts of stress-responsive genes including , , , , , and in overexpressing and silenced peach plants. Taken together, these data suggest that plays a positive role in peach response to BA stress by, at least partly, regulating ROS metabolism and stress-responsive gene expression. Our findings will be of great importance for further understanding the roles of genes in autotoxicity stress, and assist crop breeding in mitigating replant problem.

中文翻译：

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PpHSP20-26 是一种小型热休克蛋白，可增强桃子的自毒胁迫耐受性


自毒胁迫是桃再植问题的主要因素。苯甲酸（BA）被认为是重植问题中的一种关键自毒素，对桃子的生长有明显的抑制作用。据报道，小热休克蛋白（sHSP）在多种植物的多种生理和生物过程中发挥着关键作用。然而，人们对自毒性应激下的功能和潜在的生理机制知之甚少。在这里，我们识别了桃子（）并破译了它在 BA 应激反应中的作用。 BA处理显着诱导。在桃植物中过表达BA耐受性升高，而在桃植物中通过病毒诱导的基因沉默下调BA耐受性则增强了BA敏感性。与对照相比，过表达植物表现出较低的活性氧（ROS）含量和较高的抗氧化酶活性。此外，在过表达和沉默的桃植物中调节应激反应基因的转录，包括 、 、 、 、 和 。总而言之，这些数据表明，它至少部分地通过调节 ROS 代谢和应激反应基因表达，在桃对 BA 应激的反应中发挥积极作用。我们的研究结果对于进一步了解基因在自毒胁迫中的作用，并协助作物育种缓解重播问题具有重要意义。