Maternal High-Fat Diet Exacerbates Epicutaneous Sensitization and Oral Challenge-Induced Food Allergy to Ovalbumin in Offspring Mice,Journal of Agricultural and Food Chemistry

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Maternal High-Fat Diet Exacerbates Epicutaneous Sensitization and Oral Challenge-Induced Food Allergy to Ovalbumin in Offspring Mice
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2024-09-11 , DOI: 10.1021/acs.jafc.4c05373
Tianliang Bai _{1,

2,

3} , Huming Shao _{1,

2,

3} , Fan Yang _{1,

2,

3} , Xing Zhang _{1,

2,

3} , Ping Tong _{1,

2,

3} , Xuanyi Meng _{1,

3,

4} , Yong Wu _{1,

3,

4} , Hongbing Chen _{1,

3,

4} , Xin Li _{1,

2,

3}

Affiliation

Dietary factors have been associated with an increased prevalence of food allergy (FA). However, little is known about how an unhealthy diet in early life affects FA reactions in offspring. The objective of this study is to provide a scientific foundation for developing and promoting healthy dietary patterns in early life. In this study, we found that maternal high-fat diet (HFD) during pregnancy and lactation exacerbates FA (HFD-FA) in offspring mice, leading to increased serum levels of mast cell protease 1. First, we studied the systemic immunity of the HFD-FA mice and observed elevated levels of proinflammatory cytokines (IL-4, IL-6, and IL-1β) and a reduced frequency of Treg cells in splenocytes. Additionally, the HFD-FA mice showed increased gut permeability, accumulation of intestinal mast cells, and a decrease in the Treg cell frequency in the mesenteric lymph nodes. Furthermore, our findings also indicated a reduction in gut microbial diversity and abundance in HFD-FA mice. Importantly, lipid metabolism profiling revealed unique lipid profiles in the HFD-FA mice, with significant upregulation of triglycerides and downregulation of sphingolipids. Taken together, our results suggest that maternal HFD alters intestinal homeostasis and increases FA susceptibility in offspring mice.

中文翻译：

母体高脂肪饮食会加剧子代小鼠的表皮过敏和口腔刺激引起的卵清蛋白食物过敏

饮食因素与食物过敏（FA）患病率增加有关。然而，人们对于生命早期不健康的饮食如何影响后代的 FA 反应知之甚少。这项研究的目的是为发展和促进生命早期的健康饮食模式提供科学基础。在本研究中，我们发现孕期和哺乳期母体高脂饮食（HFD）会加剧子代小鼠的FA（HFD-FA），导致肥大细胞蛋白酶的血清水平升高1。首先，我们研究了小鼠的全身免疫。 HFD-FA 小鼠观察到促炎细胞因子（IL-4、IL-6 和 IL-1β）水平升高，脾细胞中 Treg 细胞频率降低。此外，HFD-FA 小鼠表现出肠道通透性增加、肠道肥大细胞积聚以及肠系膜淋巴结中 Treg 细胞频率减少。此外，我们的研究结果还表明 HFD-FA 小鼠肠道微生物多样性和丰度降低。重要的是，脂质代谢分析揭示了 HFD-FA 小鼠独特的脂质谱，甘油三酯显着上调，鞘脂下调。综上所述，我们的结果表明，母体 HFD 会改变子代小鼠的肠道稳态并增加 FA 敏感性。

更新日期：2024-09-11

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