Quantifying Phospholipids in Organic Samples Using a Hydrophilic Interaction Liquid Chromatography–Inductively Coupled Plasma High-Resolution Mass Spectrometry (HILIC-ICP-HRMS) Method,Analytical Chemistry

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Quantifying Phospholipids in Organic Samples Using a Hydrophilic Interaction Liquid Chromatography–Inductively Coupled Plasma High-Resolution Mass Spectrometry (HILIC-ICP-HRMS) Method
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-12 , DOI: 10.1021/acs.analchem.4c01883
Wladimir Ruiz _{1,

2,

3} , Stéphanie Fontagné-Dicharry ₄ , Sylvain Verdier ₅ , David C Dayton ₆ , Julie Guillemant _{3,

7} , Rémi Moulian _{2,

3} , Pierre Giusti _{1,

2,

3} , Caroline Barrère-Mangote _{2,

3} , Brice Bouyssiere _{1,

3}

Affiliation

In this study, a novel method using hydrophilic interaction liquid chromatography (HILIC) coupled with inductively coupled plasma high-resolution mass spectrometry (ICP-HRMS) was introduced for the quantification of phospholipids in oil samples. The method employed a bridged ethyl hybrid (BEH) stationary phase HILIC column with a tetrahydrofuran (THF)/water mobile phase, enhancing the solubility and detection of phospholipids. During the study, a gradient/matrix effect on ICP-HRMS sensitivity was observed and successfully compensated for experimentally, ensuring reliable quantification results. This approach has proven effective for a wide range of different oil samples including vegetable oils, animal fats, and phospholipid supplements. Notably, this method allowed the direct quantification of phospholipids in oil samples, bypassing the need for prior sample preparation methods, such as solid phase extraction (SPE), thereby streamlining the analytical process. The precision, accuracy, and reduced need for extensive sample preparation offered by this method mark a significant advancement in lipids analysis. Its robustness and broad applicability have substantial implications for industries such as food and renewable energy production, where both efficient and accurate lipid identification and quantification are crucial.

中文翻译：

使用亲水相互作用液相色谱-电感耦合等离子体高分辨率质谱 (HILIC-ICP-HRMS) 方法定量有机样品中的磷脂

在本研究中，引入了一种使用亲水相互作用液相色谱（HILIC）与电感耦合等离子体高分辨率质谱（ICP-HRMS）相结合的新方法来定量油样中的磷脂。该方法采用桥乙基杂化 (BEH) 固定相 HILIC 柱和四氢呋喃 (THF)/水流动相，增强了磷脂的溶解度和检测能力。在研究过程中，观察到了梯度/基质对 ICP-HRMS 灵敏度的影响，并成功地通过实验进行了补偿，确保了可靠的定量结果。事实证明，这种方法对于多种不同的油样均有效，包括植物油、动物脂肪和磷脂补充剂。值得注意的是，该方法可以直接定量油样中的磷脂，无需之前的样品制备方法，例如固相萃取 (SPE)，从而简化了分析过程。该方法提供的精确度、准确度以及对大量样品制备的需求减少，标志着脂质分析领域的重大进步。其稳健性和广泛的适用性对食品和可再生能源生产等行业具有重大影响，在这些行业中，高效、准确的脂质识别和定量至关重要。

更新日期：2024-09-12

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