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Label-Free Detection of Waterborne Pathogens Using an All-Solid-State Laser-Induced Graphene Potentiometric Ion Flux Immunosensor
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-12 , DOI: 10.1021/acs.analchem.4c03607 Pattarachaya Preechakasedkit 1 , Chaiwat Pulsrikarn 2 , Suphachai Nuanualsuwan 3, 4 , Nawarat Rattanadilok Na Phuket 5 , Daniel Citterio 6 , Nipapan Ruecha 1, 4
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-12 , DOI: 10.1021/acs.analchem.4c03607 Pattarachaya Preechakasedkit 1 , Chaiwat Pulsrikarn 2 , Suphachai Nuanualsuwan 3, 4 , Nawarat Rattanadilok Na Phuket 5 , Daniel Citterio 6 , Nipapan Ruecha 1, 4
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Waterborne pathogens are harmful microorganisms transmitted through water sources. Early and rapid pathogen detection is important for preventing illnesses and implementing stringent water safety measures to minimize the risk of contamination. This work introduces a miniaturized all-solid-state potentiometric ion flux immunosensor for the rapid and label-free detection of waterborne pathogens. A screen-printed silver/silver chloride electrode coated with a reference electrode membrane and polyurethane as an all-solid-state reference electrode was combined with a solid-state contact ion-selective electrode (ISE). An all-solid-state ISE was constructed on laser-induced graphene by coating it with a cationic marker and a carboxylated poly(vinyl chloride)-based membrane for immobilizing antibodies and controlling ion fluxes through the membrane. Proof-of-concept was achieved by detecting Escherichia coli and Salmonella enterica serovar Typhimurium using the assembled immunosensors within 10 min. The potentiometric response shift attributed to the blocking effect in the ion flux caused by pathogen–antibody interaction corresponded to pathogen concentration, indicating detection limits of 0.1 CFU/mL and working ranges of 0.1–105 CFU/mL. Furthermore, the developed sensors revealed high selectivity and were directly applied in groundwater and tap water without any sample preparation, demonstrating high recovery percentages. The simple operation and elimination of sample preparation are key benefits to further usability of the developed immunosensors for efficient pathogen detection.
中文翻译：
使用全固态激光诱导石墨烯电位离子流免疫传感器对水传播病原体进行无标记检测
水传播病原体是通过水源传播的有害微生物。早期和快速的病原体检测对于预防疾病和实施严格的水安全措施以尽量减少污染风险非常重要。这项工作介绍了一种小型化全固态电位离子流免疫传感器，用于快速、无标记地检测水生病原体。将涂有参比电极膜和聚氨酯的丝网印刷银/氯化银电极作为全固态参比电极与固态接触离子选择电极（ISE）相结合。全固态 ISE 在激光诱导石墨烯上构建，方法是在激光诱导石墨烯上涂上阳离子标记物和羧化聚氯乙烯膜，用于固定抗体并控制通过膜的离子通量。通过使用组装的免疫传感器在 10 分钟内检测大肠杆菌和鼠伤寒沙门氏菌，实现了概念验证。由于病原体-抗体相互作用引起的离子流阻断效应而引起的电位响应变化与病原体浓度相对应，表明检测限为0.1 CFU/mL，工作范围为0.1-10 5 CFU/mL。此外，开发的传感器具有高选择性，无需任何样品制备即可直接应用于地下水和自来水，显示出高回收率。操作简单和无需样品制备是进一步利用所开发的免疫传感器进行有效病原体检测的关键优势。
更新日期：2024-09-12
中文翻译：
使用全固态激光诱导石墨烯电位离子流免疫传感器对水传播病原体进行无标记检测
水传播病原体是通过水源传播的有害微生物。早期和快速的病原体检测对于预防疾病和实施严格的水安全措施以尽量减少污染风险非常重要。这项工作介绍了一种小型化全固态电位离子流免疫传感器，用于快速、无标记地检测水生病原体。将涂有参比电极膜和聚氨酯的丝网印刷银/氯化银电极作为全固态参比电极与固态接触离子选择电极（ISE）相结合。全固态 ISE 在激光诱导石墨烯上构建，方法是在激光诱导石墨烯上涂上阳离子标记物和羧化聚氯乙烯膜，用于固定抗体并控制通过膜的离子通量。通过使用组装的免疫传感器在 10 分钟内检测大肠杆菌和鼠伤寒沙门氏菌，实现了概念验证。由于病原体-抗体相互作用引起的离子流阻断效应而引起的电位响应变化与病原体浓度相对应，表明检测限为0.1 CFU/mL，工作范围为0.1-10 5 CFU/mL。此外，开发的传感器具有高选择性，无需任何样品制备即可直接应用于地下水和自来水，显示出高回收率。操作简单和无需样品制备是进一步利用所开发的免疫传感器进行有效病原体检测的关键优势。
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