Single-Vesicle Electrochemistry Reveals Polysaccharide from Glochidion eriocarpum Champ. Regulates Vesicular Storage and Exocytotic Release of Dopamine,Analytical Chemistry

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Single-Vesicle Electrochemistry Reveals Polysaccharide from Glochidion eriocarpum Champ. Regulates Vesicular Storage and Exocytotic Release of Dopamine
Analytical Chemistry ( IF 6.7 ) Pub Date : 2024-09-11 , DOI: 10.1021/acs.analchem.4c02493
Lijiao Cao ₁ , Jing Zhang ₂ , Mo Li ₂ , Junlan Zhou ₁ , Yuying Liu ₁ , Chunlan Liu ₂ , Xianchan Li _{1,

3}

Affiliation

Polysaccharides, which are well-known natural macromolecules, have been recognized for their protective effects on neurons and their influence on extracellular dopamine levels in the brain. It is crucial to investigate the impact of plant polysaccharides on neurotransmission, particularly regarding the vesicular storage and exocytosis of neurotransmitters. In this study, we demonstrated the possibility of studying how the polysaccharide from Glochidion eriocarpum Champ.(GPS) affects vesicle dopamine content and the dynamics of exocytosis in pheochromocytoma (PC12) cells using single-cell amperometry (SCA) and intracellular vesicle impact electrochemical cytometry (IVIEC). Our results unambiguously demonstrate that GPS effectively enhances vesicular neurotransmitter content and alters the dynamics of exocytosis, favoring a smaller fraction of content released in exocytotic release, thereby inducing the partial release mode. These significant effects are attributed to GPS’s efficient elevation of calcium influx, significant alteration in the composition of exocytosis-related membrane lipids, and enhancement of free radical scavenging ability. These findings not only establish GPS as a promising candidate for preventive or therapeutic interventions against neurodegenerative disorders but also reiterate the importance of screening native neurologic drugs with single-vesicle electrochemical approaches, the combination of SCA and IVIEC, from a neurotransmitter-centric perspective.

中文翻译：

单囊泡电化学揭示了来自Glochidion eriocarpum Champ 的多糖。调节多巴胺的囊泡储存和胞吐释放

多糖是众所周知的天然大分子，因其对神经元的保护作用及其对大脑细胞外多巴胺水平的影响而受到认可。研究植物多糖对神经传递的影响至关重要，特别是关于神经递质的囊泡储存和胞吐作用。在本研究中，我们证明了使用单细胞安培分析法 (SCA) 和细胞内囊泡冲击电化学细胞术来研究Glochidion eriocarpum Champ.(GPS) 多糖如何影响囊泡多巴胺含量以及嗜铬细胞瘤 (PC12) 细胞胞吐作用动态的可能性（IVIEC）。我们的结果明确表明，GPS 有效增强囊泡神经递质含量并改变胞吐作用的动力学，有利于胞吐释放中释放的较小部分内容，从而诱导部分释放模式。这些显着效果归因于 GPS 有效提高钙内流、胞吐作用相关膜脂组成的显着改变以及自由基清除能力的增强。这些发现不仅使 GPS 成为预防或治疗神经退行性疾病的有前途的候选药物，而且还重申了从以神经递质为中心的角度，使用单囊泡电化学方法（SCA 和 IVIEC 的组合）筛选天然神经药物的重要性。

更新日期：2024-09-11

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